Microbiology

The academic staff of department is regular participant in several national and international congress/ conferences. Research work of two boys and one girl student from Denmark was also undertaken and supervised in this department. 

The Department of Veterinary Microbiology was established since inception of the college in the year 1958 with the aim to impart undergraduate education. Initially only under graduate teaching was under taken. The postgraduate program in Veterinary Microbiology was incepted from 1995 and subsequently in Animal Biotechnology from 2007. The Ph.D program in Veterinary Microbiology was started from 2013. Currently the department offers Undergraduate, Postgraduate and Doctoral degree programs. The curriculum prepares students to meet their goals, which include professional careers in academic, clinical and technical field of Microbiology and Animal Biotechnology. So far 45 students have completed PG programs in Veterinary Microbiology and 17 in Animal Biotechnology. Four students have completed their Ph.D. and another two are undergoing Ph.D. program in Veterinary Microbiology.

The department undertakes time-bound research projects, in addition to the teaching and diagnostic services being provided for diagnosis of bacterial, viral and fungal diseases of livestock and poultry.

Heads of the Department:

Dr. S.L.Manjarekar, Dr. S.P.Phadke, Dr. M. S. Dhake, Dr. M. M. Kulkarni and Dr. R. S. Kolhe initially contributed significantly for the development of the department. Later on Dr. S. D. Harne geared up the developmental activities of the department. Subsequently Dr. D. R. Kalorey played a key role in the overall development and upliftment of the department.Recently Dr.V.C. Ingle is superannuated in March 2020 and Dr.Shubhangi Warke took the charge of Head of the Department.

Sr. No.

Name of The Head

 

Duration

1.

Dr. S.L.Manjarekar

1958-1964

2.

Dr. S.P.Phadke

1964-1969

3.

Dr. P.R.Dhake

1969-1975

4.

Dr. R. S. Kolhe

1975-1980

5.

Dr. S. D. Harne

1980-1997

6.

Dr. D. R. Kalorey

1997-2014

7.

Dr. V.C.Ingle

2014-2020

8.

Dr.Shubhangi Warke

2020-till date

 

Present status of the Department

A) Staff position (Teaching and Non –teaching)

Veterinary Microbiology:

Position

Name of faculty

Qualification

Professor

I/c Dr.Shubhangi Warke

M.V.Sc., Ph.D.

Associate Professor

Assistant Professor

Dr.Shubhangi Warke

M.V.Sc., Ph.D.

Assistant Professor

Dr.P.A. Tembhurne

M.V.Sc., Ph.D.

Sr. Laboratory  Technician

Dr. Jiwan Kesarkar      

M.V.Sc.

 Animal Biotechnology

Position

Name of faculty

Qualification

Assistant Professor

Senior Research Fellow

Vacant

 

B)  Infrastructure facilities:

i) Laboratories:

Sr. No.

Name of Laboratory

Purpose

1.

Bacteriology and Mycology Laboratory

For carried out separate  work of Bacteriology and Mycology.

2.

Veterinary Immunology Laboratory

For carried out separate  work of Immunology

3.

Virology Laboratory

For carried out separate  work of Virology

4.

Molecular Diagnosis Laboratory

For carried out molecular biology work

 

ii) Equipments available for Teaching and Research

Sr. No.

 Instruments available for Research

 Availability

1.

Laminar flow  (Vertical /Horizontal)

 3

2.

Binocular Microscope

 3

3.

Deep fridge ( -200C)

 4

4.

Thermal cycler (PCR machine)

 2

5.

Gel Documentation system

 1

6.

CO2 Incubator

 1

7.

Inverted Microscope

 1

8.

Refrigerated centrifuge

 2

9.

Deep freezer ( -800C)

 1

10.

ELISA reader

 1

11.

Spectrophotometer

 1

12.

Real Time PCR

 1

13.

Gel Electrophoresis apparatus

 2

14.

Lyophilizer

 1

15.

Hybridization oven

 1

 



Dr.Shubhangi Warke (Bhagat)
(MVSc, Ph.D., DBM, NET)
Assistant Professor
Phone – (O) 0712 2510087,
(Fax) 0712 2510883, (M) 9561214395
email : shubhangi_warke@rediffmail.com

Field of specialization : Veterinary Microbiology

 Teaching

  • *UG and PG teaching in Veterinary Microbiology
  • *PG teaching in Animal Biotechnology
  • * UG teaching in Fishery College
  • *Laboratory manuals prepared for Veterinary Microbiology  UG  & PG Teaching,

          *Paper setter for UG theory examinations for different universities

          *Participation as External Examiner for different universities.

  • *Guiding students for ICAR Entrance and interviews

Research:

MVSc research: STUDIES ON EFFECT OF AQUEOUS  HERBAL EXTRACTON GROWTH OF ASPERGILLUS OCHRACEOUS, OCHRATOXIN PRODUCTION AND ITS NEUTRALIZATION

Ph.D. research: Genotypic and Phenotypic Characterization of Listeria monocytogenes from bovines and their environment.


P.G. Students Guided:

Major Guide in Veterinary Microbiology: 02

Member of advisory Committee in Veterinary Microbiology

& Animal Biotechnology: 15

Member of advisory committee in other disciplines: 03

 

Research Projects:.

Completed Research projects:

Title of Project

Funding agency

   Amount in Lakhs

 Date of Sanction and Duration

Genetic Diversiy of Listeria monocytogenes with different virulence potential
( Principle Investigator)

DST

10.27200/-

2010 -2013

Virulence profiling of Listeria monocytogenes isolated from food-, veterinary and clinical sources
(Co-
 Investigator)

ICMR(Indo German)

10.00/-

2011-2013

ranslational research on Molecular epidemiology of L.monocytogenes
(Co-
 Investigator)

CEIB

(DBT)

50.00/-

2012( 5 years)

DBT Network Project on Brucellosis
(Co- Investigator)

DBT

1,14,699/-

2012 (5 years )

 

Completed Sponsored Project

Title of Project

Funding agency

 Amount  in Lakhs

       Date of  sfdfdgfdgfdgdfggfggfgf    Sanction and Duration

To study the safety & immunogenicity of Brucella abortus RB51, Vaccine in cattle

(Co-PI)

IIL

48,750/-

      1st to 31st November,2010

Evaluation of  mastodon in bovine mastitis

(CO-PI)

Cattle Remedies India Ltd.

80,040/-

 January, 2012 to August ,2012

An open label multicentric clinical trial to study safety and immunogenicity of Tetanus toxoid vaccine in animals

Indian Immunologicals

2.29200/-

March 2019-

.

PUBLICATIONS :

1) Scientific research paper published in International Journals : 11   
2) Scientific research paper published in National Journals : 42
3) Research paper presented at International Seminar :09
4) Research paper presented at National  Seminar :86
5) Popular article : 42

ACHIEVEMENTS:

*    Identified herbs with antiochratoxic & OA neutralizing activity
*   Developed Milk based  Dot ELISA for detection of   L. monocytogenes
*    Developed Milk based Indirect ELISA for detection of   L. monocytogenes
*    Developed Milk based  AB ELISA for detection of   L. monocytogenes
*    Developed Serum based  Indirect  ELISA for detection of   L. monocytogenes
*   Isolation & characterizations of Listeria from wild life
*   Antibacterial activity of herbal extracts
*   Developed multiplex PCR for detection of virulence marker genes of Listeria spp.
*  Identify Disinfectant  to reduced contamination of L. monocytogenes at bovine farm.
* Developed Multiplex PCR for detection of antigen of ParvoVirus
*  Gene Sequence  submitted  to gene bank (NCBI) : 55

 

 Recommendation:  Research Recommendation -01 in 2019

AWARDS & HONORS:

 1.Fellowship :Dr. B.V. Rao WPSA (IB) WPC, 1996. Research Grants 2000 for Post Graduate Research Program.

 2. Ayurvet Best Research Article – IX Annual Conference of IAAVR , 2002.

 3. Best Poster award – IX Annual Conference of IAAVR, 2002

 4. Young Scientist Award -2009 for outstanding service in the field of Veterinary Science

 5. World Environment Educational award -2012

 6. Mother Teresa Sadbhawana Award-2015

 7.Certificae of Appreciation -2016 International Womans Day

 8.Appreciation Certificate  for participated to organized XXXth annual Convention& National Symposium of IAVMI at Nagpur from 10-12 th February ,2017.

 9.Letter of Appreciation in recognition as  a Member of Convocation Cell of eighth convocation of Maharashtra Animal & Fishery Sciences University held during 9 th March ,2017

 10 Best poster award– First best Poster Presentation Award during XV Biennial Conference and National Symposium of IAWV at Hyderabad  during 21-22 ,November, 2017.

 11.First Prize for oral Presentation at National Symposium  at Akola during 26th -27th November,2019.

 12 Second Prize for poster presentation at  National Symposium at Akola during 26th -27th November,2019.

 13 Letter of Appreciation:  for worked as Member in judging Committee for Elocution & Drawing Competition held on the occasion of “World Animal Day” on 4th October, 2019,  at Nagpur Veterinary College, Nagpur .

 14. Appreciation Certificate for outstanding contribution and dedicated efforts for successful organization of National conference & XVI Annual Symposium of IAVPHS on 26 and 27 th February,2019 at NVc., Nagpur

 15.Certificate of Honour for Best Paper Presentation award in Canine Microbiology & Immunology Session 2019   In XVII National Congress on Canine Practice & Natiional Symposium  at Jabalpur(M.P.)  during 21-23 Jan 2020.

Organization of Event:

1.Organized 30th Annual Convention of Indian Association of Veterinary Microbiologists Immunologists and Specialist in Infectious Diseases (IAVMI) and National Symposium on “Challenges in Animal Health for Higher productivity and Income to farmers” during 10-12th Feb 2017.——- as  Joint secretary

2. Associated with Training and Workshop organized by Department:08

 

EXTENSION: 

 Extention and Field Outreach Activities : Technical guidance to farmers and Field Veterinarians through camps, lectures, trainings, radio talk , T.V. talks  & visit at adopted villages etc.

Dissemination of technical knowledge through article publications in local news paper , leaflets & pamphlets

  

MEMBERSHIP:

Membership of Scientific organizations (8),

1) Life member of Indian association of Veterinary Microbiologist,Immunologist and specialist in Infectious Diseases.(IAVMI)
2) Life Member of Indian association for the Advancement of Veterinary Research (IAAVR)
3) Life member of Indian association of Veterinary Public Health Specialist (IAVPH),
4) Society for Immunology &Serology
5) Indian Veterinary Community
6)   Life member of  National association  for welfare of Animal Research
7) Life Member of Society for Listeria
8)   Life Member of Indian Association for Woman’s  Veterinarians’  (IAWV
9)  Life Member of  Indian Society of Canine  Practitioner


Dr. Prabhakar A Tembhurne,
(MVSc, PhD, PDF(USA))<
Assistant Professor
Phone – (O) 0712 2510087, (Fax) 0712 2510883, (M) +91 9765712029
email:prabhakar.tembhurne@gmail.com

 

Field of specialization: Veterinary Virology

Field of Interest

small RNA biology (miRNA),  Molecular diagnostics &  epidemiology  of animal pathogens, Host-pathogen interaction.

Teaching

UG ,PG and PhD  teaching in Veterinary Microbiology using audiovisual and Web based  tools.

PG teaching in Animal Biotechnology using audiovisual and Web based  tools

Manuals prepared for Animal Biotechnology and Veterinary Microbiology courses.

 

Research

M.V.Sc. (IVRI)  Research – Studies on the development of inactivated monovalent vaccine against Bluetongue virus.

Ph.D.  (IVRI) Research – Molecular characterization of L2 and M5 genes of Bluetongue virus serotypes 18 and 23.

Post Doctoral Research  2004-2007 (North Carolina State university, Raleigh ,USA):     

Functional Genomics: miRNA profiling in developmental chicken embryo: Deciphering role of antimeq gene in MDV pathogenesis

Research Scientist(National AIDS Research Institute, Pune):

HPTN/ACTG clinical monitoring of Viral Load in HIV patients

 

Post-graduate Student Guided:
Major advisor: PG (M.V.Sc)  In Veterinary Microbiology) : 03
PG (M.V.Sc ) In Animal Biotechnology)    : 06
MSc : 02
PhD In Veterinary Microbiology: 01
Member of Advisory: PG (M.V.Sc ) : 35 PhD : 5

Research Project:

Title of Project

Funding Agency

Amount

Date of sanction and Duration

Completed Projects:

Principle Investigator

Understanding the molecular basis

of peste-des-petits ruminants virus

(PPRV) mediated host immune

modulation of the development of

next generation vaccines

 NASF- ICAR, Govt. of India, New Delhi

49.12 lakhs

2017-20

Co- Investigator: (Lead Centre PI

& Coordinator in 2020)

“Molecular Surveillance and Cross

Species Transmission of Rotaviruses

from Animal and Human Origin

from Maharashtra

DBT, Govt. of India,New Delhi

50 lakhs

2017-20

Co-Investigator:

“Centre for Zoonosis

ICAR-ICMR,Govt. of India, New Delhi

437.608 lakhs

2015-2017

Co- Investigator:

“Molecular epidemiology of Brucellosis”

 

DBT, Govt. of India, New Delhi

114 lakhs

2012-15

 

Principle Investigator

Deciphering role of miRNA in chicken embryonic developmental pathology and its evaluation as therapeutics in immune suppressed chicks.

DST, Govt. of India, New Delhi

 

20 Lakhs

2012-15

Co- Investigator:

Epidemiological studies on classical swine fever in central India & Goa.

DBT, Govt. of India, New Delhi

105 Lakhs

2009-2013

 

Research Achievements:

1) Research Recommendations : 03

2) Published Full Genome of IBDV circulating in Nagpur Region

3) Developed NEWS paper/ordinary Filter based DSS transport & detection methodology

for the diagnosis of Peste-des-petits-ruminants virus.

4) Identified novel miRNAs in PPRV infections and miRNAs as prognostic biomarker for

PPRV infections.

5) Deciphered microRNAs (miRNA) role in embryonic immune organ developmental

pathology by Knockdown miRNA & in immune competency in immune suppressed

chicks by therapeutic miRNA.

6) Identified novel microRNAs in developing chicken embryos and in immunological important organs in chicken.

7) Contributed for development of Recombinant protein based ELISA (Launched by ICAR) for diagnosis of Bluetongue virus (BTV).

8) First to define the emergence of polyclonal escape mutant of BTV leading to thrust for adaptation of molecular tools for characterization of BTV over serological typing.

9) First to published full length genetic makeup important viral genes of BTV serotypes 18 & 23

10) First to develop a successful BEI inactivated experimental vaccine for BTV Indian isolates. 


Awards & honors:

Recipient of BHARAT RATNA SIKSHA AWARD conferred by Global Society for Heath & Educational Growth, New Delhi.

Best Poster Award :-IAVMI 2019.


Publications:

Research Publications:  International (Peer reviewed Journals): 7, National 28  

Other Publications/Presentations in Conferences/Seminars –

International: 9 National: 40

Popular articles /manuals  etc: 5

Gene sequences Submitted: 54


Member of Scientific Society:

Indian Association for Advancement of Veterinary Research

Indian Association for Veterinary Immunolgy and Microbiology 


Extension:

Extension and Field Outreach Activities: technical guidance to farmers, field veterinarian through camps, lectures, trainings & visits at adopted villages etc.

Dissemination of technical knowledge through extension publications in local language, manuals etc.

Diagnostic Service : For animal diseases diagnosis for viral,bacterial and fungal disease

Currently in COVID-19 diagnosis at ICMR approved NVC,Nagpur Lab.


Extension project : 01

Title of Project

Funding Agency

Amount ( lakhs)

Date of sanction and Duration

Completed Project:

Co-Investigator:

Establishment of community based Animal Husbandry Practices in Tribal Village of Gadchiroli District of Maharashtra State

ICAR, New Delhi

36.33 lakhs

2014-15

  •  Achievements of the Department:

    • Infrastructure development for Education and Research in Veterinary Microbiology & Animal Biotechnology (UG,PG & PhD)
    • Development of HRD in Animal Biotechnology through national programme.
    • Establishment of Laboratory Animal Experimental Facility.
    • Repository of bacterial & viral isolates viz. Staphylococcus aureus, Listeria monocytogenes, E. coli and Parvovirus, Poxviruses, IBD etc
    • Development of in-house assays for diagnosis of infectious diseases viz. Sample collections (Dried spot sample PCR using newspaper /filterpapers) –DSS PCR); Multigene based identification of PPRV for better confirmation of disease in Indian scenario).
    • Antigen detection of Parvovirus by Multiplex PCR
    • Molecular epidemiology of Staphylococcus spp., Listeria, spp. Rotaviruses and Poxviruses, PPR, and IBD from Maharashtra State.
    • Identified herbs for neutralization and inhibition of aflatoxin and ocrotoxin A.
    • Deciphered role of miRNA in therapy of immunocompromized chicken.
    • Development of Milk (Dot, Indirect & AB) and Serum(Indirect) ELISA for detection of L. monocytogenes
    • Isolation & characterizations of Listeria from wild life
    • Identify Disinfectant  to reduced contamination of L. monocytogenes at bovine farm.
    • Several Gene Sequence  submitted  to gene bank

     Facility created:

    • High end research facilities viz. equipments ( Real time PCR, Thermal Cyclers, Hybridization oven, Electrophoresis systems,  Biosafety cabinets, CO2 incubators,   etc)
    • Establishment of Laboratory Animal Experimental Facility.
    • Cell culture facility for virology works
    • Molecular biology facility ( DNA/RNA/Protein works)

     

    Scientific Collaborations 

    • Central India Institute for Medical Sciences, Nagpur (MOU signed)
    • Apex research institutes in Veterinary Sciences, like IVRI, Izatanagar, NIVEDI, Bangalore, National Institute of Virology, Pune etc
    • Indira Gandhi Government Medical College, Nagpur
    • Government Medical College, Nagpur
    • Mahatma Gandhi Institute of Medical Sciences, Sewagarm, Wardha
    • Veterinary Colleges of different states
    • IISER, Mohali
    • Delhi University (South Campus)
    • Indian Immunologicals
  1. Vision/Mission  of the Department:

    To impart cutting edge U.G. and P.G. and Ph.D. teaching to create efficient Human Resource in Veterinary Microbiology and Animal Biotechnology

    To establish Centre for Animal Disease Diagnosis and Research

    To establish technical linkage with national and international scientific organizations for better academics and research.

    To explore research in functional genomics especially in small RNA biology and its application for future therapeutics

    To cater services to state government officers, livestock owners through extension activities.

  1. Course Offered / Conducted by the Department:

     U.G.  course :  Veterinary Microbiology ( MSVE -2016)  3+2=5 II nd Year of BVSc.& AH

     P.G.Courses: (In Vety.Microbiology)

    Sr. No.

    Corse  No.

    Title

    Credits

    Semester

    1

    VMC-601

    Bacteriology -I

    3+1=4

    I

    2

    VMC-602

    Bacteriology -II

    3+1=4

    II

    3

    VMC-603

    Veterinary Mycology

    1+1-2

    II

    4

    VMC-604

    General Virology

    2+1=3

    I

    5

    VMC-605

    Systematic Animal Virology

    3+1=4

    II

    6

    VMC-606

    Principles of Immunology

    2+1=3

    I

    7

    VMC-607

    Vaccinology

    2+0=2

    III

    8

    VMC-608

    Diagnostics of Infectious Diseases

    1+2=3

    III

    9

    VMC-609

    Techniques in Microbiology & Immunology

    0=3=3

    I

    10

    VMC-691

    Masters Seminar

    1+0=1

    III

    11

    VMC-699

    Masters Research

    20

    III & IV


    P.G. Courses : (In Animal Biotechnology)

    Sr. No.

    Corse  No.

    Title

    Credits

    Semester

    1

    ABT-601

    Basic & Applied  Biotechnology

    3+0=3

    I

    2

    ABT-602**

     Fundamentals of Cell & Molecular Biology

    3+0=3

    I

    3

    ABT-603

    Applied Molecular Biology

    2+1-3

    II

    4

    ABT-604**

    Animal Cell Culture; Principles & Applications

    1+2=3

    I

    5

    ABT-605**

    Molecular Diagnostics

    1+2=3

    II

    6

    ABT-606

    Vaccine Biotechnology

    2+0=2

    III

    7

    ABT-607

    Immunology Applied to Biotechnology

    1+1=2

    III

    8

    ABT-608

    Introduction to Bioinformatics

    1+1=2

    III

    9

    ABT-609**

    Animal Genomics

    2+1=3

    II

    10

    ABT-610**

    Reproductive Biotechnology

    2+1=3

    III

    11

    ABT-611**

    Techniques in Molecular Biology and Genetic Engineering

    0+3=3

    II

    12

    ABT-613

    Molecular Forensics

    2+1=3

    III

    13

    ABT-616

    Animal Biotechnology

    0+3=3

    II

    14

    ABT-691

    Masters Seminar

    1+0=1

    III

    15

    ABT-699

    Masters Research

    20

    III & IV

    ** Compulsory courses

    Ph.D.Courses: (In Vety.Microbiology)

    Sr. No.

    Corse  No.

    Title

    Credits

    Semester

    1

    VMC-701

    Advances in Bacteriology

    2+1=3

    I

    2

    VMC-702

    Advances in Mycology

    2+1=3

    I

    3

    VMC-703

    Bacterial Genetics

    2+1=3

    I

    4

    VMC-704

    Microbial Toxins

    2+1=3

    II

    5

    VMC-705

    Molecular Determinants of Bacterial Pathogenesis

    2+1=3

    II

    6

    VMC-706

    Advances in Virology

    2+1=3

    I

    7

    VMC-707

    Molecular and Genetic Aspects of Viral Pathogenesis

    2+1=3

    II

    8

    VMC-708

    Structure Function Relationship of DNA and RNA Viruses

    3+0=3

    II

    9

    VMC-709

    Oncogenic Viruses

    2+0=2

    III

    10

    VMC-710

    Slow Viral Infections and Prions

    2+0=2

    III

    11

    VMC-711

    Molecular Immunology

    2+1=3

    I

    12

    VMC-712

    Advances in Cellular Immunology

    2+1=3

    I

    13

    VMC-713

    Cytokines and Immunomodulators

    2+0=2

    II

    14

    VMC-714

    Advances in Vaccinology

    2+0=2

    III

    15

    VMC-715

    Advances in Immunodiagnostics

    1+1=2

    III

    16

    VMC-716

    Modern Immunotechnology

    1+2=3

    III

    17

    VMC-717

    Current Topics in Infection and Immunity

    3+0=3

    III

    18

    VMC-718

    Veterinary Microbial Biotechnology

    2+1=3

    II

    19

    VMC-790

    Special Problem

    0+2=2

    IV

    20

    VMC-791

    Doctoral Seminar I

    1+0=1

    II

    21

    VMC-792

    Doctoral Seminar II

    1+0=1

    III

    22

    VMC-799

    Doctoral Research

    45

     

Departmental Research Activity :

Research programmes:

i) Completed Research Projects

Sr. No.

Type

Principle

Investigator

Title

Period

Cost

(Rs in Lakhs )

1.

NATP(MM)

Dr. D.R.Kalorey

Dr.V.C.Ingle

Animal Health information system through disease monitoring and surveillance

2000-04

19.80

 

2.

NATP(CGP)

Dr. D.R.Kalorey

Dr.V.C.Ingle

Development of ELISA for detection of L. monocytogenes from milk.

 

2002-05

14.09

3.

DBT Network

Dr. D.R.Kalorey

Development of Standardized herbal product for bovine mastitis

2006 -11

43.22

4.

DST(SERB- Young Scientist)

Dr.S.R. Warke

Genetic diversity of Listeria monocytogenes with different virulence potential  

2010-13

10.00

5.

Indo German ICMR

Dr.D.R.Kalorey

Dr.N.V.Kurkure

Dr.S.R. Warke

Virulence profiling of Listeria monocytogenes isolated from food, veterinary and clinical sources.

2011-13

10.66

6.

DBT

Dr.D.R.Kalorey

Dr. N.V. Kurkure

Dr. P.A. Tembhurne

Dr.ShubhangiWarke

Dr. S.P.Awandkar

DBT Network Project on Brucellosis.

 

2012-16

114.70

7.

DST

Dr. P.A. Tembhurne

Deciphering role of miRNA in chicken developmental pathology & its evaluation as therapeutic in immune suppressed chicks.

2012-15

18.00

8.

DBT-HRD

Dr.V.C.Ingle

Development HRD in Animal Biotechnology.

2010-onwards

109.02

9.

DBT

Dr.D.R.Kalorey

Dr.N.V.Kurkure

Dr. S. R. Warke

Translational research on Molecular epidemiology of L. monocytogenes.

 

2012-2017

57.70

10.

ICAR-TSP

P. A. Tembhurne

( Co-PI)

Establishment of community based animal husbandry practice in tribal villages of Gadchiroli district of Maharashtra State

 

2014-2017

206.00

11

ICAR-ICMR

Dr.V.C.Ingle

( Co-PI)

P. A. Tembhurne

( Co-PI)

Dr.S.P.Awandkar

( Co-PI)

Centre for Zoonoses

(Niche Area of Excellence)

2015-2019

407.00

12

ICAR NASF

Dr. P.A.Tembhurne  (Co-operating centre PI)

Understanding the molecular basis of peste-des-petits ruminants virus (PPRV) mediated host immune modulation for the development of next generation vaccines

2016-2019

300.00

13

ICMR

 Dr. V.C. Ingle

Molecular  epidemiology and cross species transmission of rotavirus of animal and Human origin from Maharashtra

2017-2020

70.00

  

ii)Ongoing Research Projects:

Sr.

No.

Type

Principle Investigator

Title

Period

Cost (Rs in lakhs)

1

DBT

Department of Veterinary Microbiology

Star College Scheme of DBT

2020-2022

19.00

 

ii) Research Projects submitted to various funding agencies

Sr. No.

Title of Project

Investigators

Duration/

Date of submission

Funding Agency

Total Outlay

(in lakhs)

1.

Molecular epidemiology & development of multivalent vaccines against Bovine Mastitis caused by Staphylococcus aureus

Dr.D.R.Kalorey

3 yrs

MBC

221.70

2.

Development of Multiple Dot –ELISA for simultaneous diagnosis of reproductive diseases.

Dr.D.R.Kalorey

3 yrs

MBC

102.21

3.

Molecular characterization of Avian pathogenic Escherichia coli  

Dr.D.R.Kalorey

3 yrs

MBC

192.10

4.

Molecular characterization of Brucella strains from reproductive disorders of  cattle, buffalo and goat

Dr. V.C.Ingle

3 yrs

MBC

90.56

5.

Development of standardized vaccine for Staphylococcal bovine mastitis in India.

Dr.D.R.Kalorey

3 yrs

BIPP

12.119744 Crores (2.747624 crores)

6.

Development of vaccine against bovine mastitis caused by CNS

Dr.D.R.Kalorey

3 yrs

RGS& T C

67.14

7.

Development of multiplex PCR for detection of enterotoxigenic Staphylococcus aureus from milk and milk products  

Dr.D.R.Kalorey

3 yrs

RGS& T C

59.14

 

8.

Molecular Epidemiology and development of vaccine against bovine mastitis

Dr.D.R.Kalorey

3 yrs

DBT Network

612.24

9.

Central Disease Diagnostic Laboratory

Dr.D.R.Kalorey

3 yrs

 

ICAR

56.00

 

10.

Deciphering role of miRNA in chicken embryonic developmental pathology and its evaluation as therapeutics in immune suppressed chicks.  

Dr. P.A.Tembhurne

3 yrs

DST-FAST TRACK

24.672

11.

Evaluation of antiviral activity of Growell liquid against Fowl pox virus.

Dr. P.A.Tembhurne

6 months

 

Growell India

1.20

12.

Centre for Animal Disease Diagnosis, Research and Control

Dr.D.R.Kalorey

5 Years

RKVY

461.50

13.

Safety and immunogenicity of Brucella abortus RB51 vaccine in  cattle and buffaloes

 

Dr.D.R.Kalorey

3 Years

DBT

55. 648

14.

Development of serological and DNA based diagnostic tools for brucellosis

Dr.D.R.Kalorey

DBT

DBT

71.686

15.

*miRNA profiling  of canine  transmissible venereal tumor : A novel markers  in body fluids for early diagnosis of cancer

Dr. P. A. Tembhurne

Dr.V.C.Ingle

Dr. S. P. Awandkar

Dr.G.R.Bhojne

 

3Yrs

DBT

32.00

16

Molecular markers to identify pathogenic Listeria monocytogenes from animal food origin & human clinical cases.

Dr. S.R. Warke

Dr.V.C. Ingle

Dr. N.V. Kurkure

3 Yrs

11-4-2014

DBT

48.84

17

Development of Infrastructure Facility for Animal Disease Diagnosis and Molecular Characterization of Pathogens

Dr.V.C.Ingle

Dr. P. A. Tembhurne

Dr. S. P. Awandkar

Dr.U.M.Tumlam

Dr.S.R.Warke

Dr.V.K.Basunathe

Dr.G.R.Bhojne

Dr.P.M.Sonkusale

3Yrs

13-10-2015

RKVY

282.8

18.

*Decipering molecular interaction of Infectious Bursal Disease Virus (IBDV) with cellular genes and host encoded miRNA

Dr. P. A. Tembhurne

 

21-10-2015

ICAR

 

19.

Studies on Molecular Epidemiology of Pestiviruses in small ruminant

Dr.V.C.Ingle

2 yrs

11-9-2015

 

 

 

 

ICAR Ad-hoc

40 .00

20.

Epidemiology and Impact Assessment Of Infectious Bovine Rhinotrachitis in Livestock of Central India

Dr.S.P.Awandkar

 

2 yrs

11-9-2015

 

 

 

ICAR Ad-hoc

40 .00

21.

Studies on cross species transmission of Rotaviruses in livestock

Dr.V.C.Ingle

6 m

8-2-2016

NAAS short term study

1.00

22

Disinfectant Resistant of L.monocytogens from bovine environment

Dr.S.R.Warke

6 m

8-2-2016

NAAS short term study

1.00

23.

Status of clean milk production at organized and non-organized farms in Bhandara district

Dr.S.P.Awandkar

6 m

8-2-2016

NAAS short term study

1.00

24.

Curing of plasmid from CPS & CNS isolated from Bovine Subclinical Mastitis using Kamdhenu ark

Dr.U.M.Tumlam

6 m

8-2-2016

NAAS short term study

1.00

25.

Evaluation of cheapest cellular matrix base materials viz. News paper/filter paper for room temperature PPR sample dispatch and its molecular characterization

Dr.P.A.Tembhurne

6 m

8-2-2016

NAAS short term study

1.00

26.

Holistic approach to identify common infectious agents of diarrhea in dogs

Dr.P.A.Tembhurne

21-6-2016

DBT

 

27.

Studies on Molecular epidemiology of IBR virus in cross bred bovines of Maharashtra state

Dr.V.C.Ingle

3 Years

11-7-2016

RGSTC

20.00

 

 Agency Projects undertaken by the Department 

Sr. No.

Agency

Principle

Investigator

Title

Period

Cost (Rs)

1.

Growell India Pvt.Ltd. Pune

Dr.D.R.Kalorey

Role of Growell as immuno modulator in IBD vaccinated Chicks.

1997

18,950/-

 

2.

Growell India Pvt.Ltd. Pune

 

Dr.D.R.Kalorey

Immunomodulatory effect of Growell during experimental Aflatoxicosis in chicks.

1997

26,880/-

 

3.

DaburAyurvet Ltd. Gaziabad -10

Dr.D.R.Kalorey

Effect of herbomineral premix on fungal Growth & in vitro inhibition of aflatoxin B1 production.

1998

30,500-/

 

4.

DaburAyurvet Ltd. Gaziabad –10

Dr.D.R.Kalorey

Effect of herbomineral toxin binder during experimental mycotoxicosis in broiler chicks.

2000

30,500/-

 

5.

Kemin Nutriti onal Technolo gies,(India)

Dr.D.R.Kalorey

Efficacy of Toxin-binder against aflatoxicosis in  broilers.

2002

30,000/-

6.

Dabur Ayurvet Ltd

Gaziabad – 10

Dr.D.R.Kalorey

In vivo efficacy of herbal preparation during simultaneous mycotoxicosis in Broilers.

2002

 15,000/-

 

7.

Dabur Ayurvet Ltd

Gaziabad – 10

Dr.D.R.Kalorey

.Evaluation of efficacy of mastilep during subclinical mastitis

2002

10,000/-

8.

M/s Intervet Pvt. Ltd.,Pune    

Dr.D.R.Kalorey

Evaluation of Brucella RB 51               vaccine under field condition.   

2005    

60,000/-

9.

M/s Intervet Pvt. Ltd.,Pune    

Dr.D.R.Kalorey

Evaluation of safety and efficacy of oil and gel adjuvanted BOVILS (R) HSBQ vaccine in cattle under field condition.

2007

25,000/-

10.

Bentoli Agri Nutrition Asia Pvt. Ltd

Dr.D.R.Kalorey

Evaluation of antibacterial and antifungal activity of antimicrobial product.

2007

1,65,865/-

11.

Bentoli Agri Nutrition Asia Pvt. Ltd

Dr.D.R.Kalorey

Antifungal and antimycotoxic activity of mold inhibitor

2007

38,080/-

12.

Indian Immunologicals Limited

Dr.D.R.Kalorey

To study Safety and immunogenicity of Brucella abortus RB51  in  cattle

2009

48,750/-

13.

Cattle Remedies, India Ltd.

Dr.D.R.Kalorey

Evaluation of Mastodin in bovine mastitis

  2011

80,000/-

14

IIL,

Hyderabad

Dr.S.R.Warke

An open label multicentric clinical trial to study safety and immunogenicity of Tetanus toxoid vaccine in animals

2019-2020

2.29

 

 

Recommendations:

Sr. No.

Project No.

 

Research Workers

Recommendations

 1

NVC/Micro/02/2004

Dr. D.R.Kalorey

Dr.Jyoti Bodade

Chicks vaccinated at 7th day of age with inactivated emulsified ND+IBD vaccine containing  10%  &  20% Vit E are protected  against New Castle Disease.

 

 2

NVC/Micro/01/2007

Dr. D.R.Kalorey

Dr.P.K.Jallewar

Norfloxacillin & Chloramphenicol can be used in Listeria infection, since the prevalence of Listeria species  in fresh water fish Vagur (Clavrus batracus) was 19.50% and of Listeria monocytogenous  13.00 per cent.

 

 3

NVC/Micro/02/2007

Dr. D.R.Kalorey

Dr. Deepti  K.Pillai

Antigen capture Sandwich ELISA developed for the detection of Pasteurella multocida antigen is sensitive (81.25%) specific ( 100%) and easy to perform, hence can be used as an efficient diagnostic tool for the diagnosis of fowl cholera from serum.

 

 4

NVC/Micro/02/2015-16

Dr. P.A Tembhurne

Dr. M.A.Pundlik

Ink free news paper or filter paper of around 1.5cm diameter spotted with nasal secretion of affected animals was found suitable for diagnosis of Peste des Petits Ruminants Virus   by RT-PCR.

 

 5

NVC/Micro/02/2016-17

Dr. P.A Tembhurne

Dr. R. Narayan

For Detection of PPRV (Peste des Petits Ruminants Virus) in field samples, RT-PCR  technique targeting both the M & N gene should be employed.

 6

NVC/Micro/     /2018-19

 

 

Dr.Shubhangi Warke                       

The disinfectant Didocyldiamethyl ammonium chloride (DAC), found to be most effective against the L. monocytogenes, lower that the recommended concentration

 

 

 7

NVC/Micro/     /2018-19

 

 

 

Dr. P.A Tembhurne

Dr. Pooja Acharya  

The  multiplex PCR approach  for amplification of cytb of tiger & leopards   and its sequencing  from scat sample easily obtained  sample in wild as well as captivity will be a tool  useful for identification  of endangered species

 

Publications:

1) Scientific research paper published International    : 11, National   : 127

2)Research paper presented at seminar International  : 15, National :117        

3)Popular article : 42

4) Prepared Manual  on  Milk Dot ELISA for detection of    L. monocytogenes.

5)  Prepared Manual  on  Milk  Indirect  ELISA for detection of    L. monocytogenes.

6) Prepared Manual  on  Milk Avidin Biotin  ELISA for detection of    L. monocytogenes.

7) Prepared Manual  on  Serum ELISA for detection of    L. monocytogenes. 

8) Prepared SOPs of  Diagnostic protocol

9) Prepared Monograph: 05

10) Prepared Leaflets:10

 

B) Area of Research

Seminar/Conference / Organized:

Sr. No.

Seminar/Conference /Training organized

Date

1.

IX Annual Conference of Indian Association for the Advancement of  Veterinary Research (IAAVR) and National Symposium on “Concepts in Sustainable Livestock Production  and Health in New Millennium” 

4-5th Feb 2002

 

2.

 

National symposium on “Zoonoses and Biotechnological Applications”

 

4-5th  Feb.2008

 

3.

30th Annual Convention of Indian Association of Veterinary Microbiologists Immunologists and Specialist in Infectious Diseases (IAVMI) and National Symposium on “Challenges in Animal Health for Higher Productivity and Income to Farmers”

 

10-12th Feb 2017

 

 

Training/ Workshop/Popular Lecture

 

Sr. No.

Seminar/Conference /Training organized

Date

1.

Popular Lecture Series on Biotechnology.

 

4th  Oct.2007

2.

Work shop on “Dairy Management & Nutrition”

 

24th Aug. 2007

3.

Short term training course on “Application of Molecular Methods in Livestock Disease Diagnosis and Research”

 

17th-31st  July,2008

 

4.

 Short term Training course on “Diagnosis of Brucellosis” under the Continuing Veterinary Education Programme funded by Veterinary Council of India.

 

25-28th May 2009

5.

Short term Training course on “Diagnosis of Brucellosis” under         the Continuing Veterinary Education Programme funded by Veterinary Council of India,  New Delhi.

 

1-4th June 2009

 

6.

 

Three day workshop on “Care, Breeding and Management of Laboratory Animals.

 

15-17th Jan 2009

 

7.

Work shop on Bioinformatics and PCR. 

19-20thMarch 2010

 

8.

One day workshop on ”Dugdhotpadan: Krishi Jivnacha Shashwat Aadhar.

 

26th Dec 2014

9.

Entrepreneurial Seminar on “Large scale production of anti snake venom”

 

6th June 2015

 

 

Diagnostic services catered 

Diagnostic services are catered on payment basis with the following charges.

 Sr.No.       

Diagnostic Tests

Amount (Rs)

  1.

Cultural Examination & AST

200.00

  2.

Biochemical characterization of bacteria

200.00

  3.

Smear examination

100.00

  4.

Skin/hair for mycotic infection

300.00

  5.

Mycotoxin in feed

500.00

  6.

Brucellosis by RBPT/Salmonellosis by PAT

50.00 / Sample

  7.

Brucellosis by STAT

100.00/ Sample

  8.

Brucellosis by ELISA

500.00/ Sample

  9.

Tuberculin/Johnin test

200.00 / animal

  10.

Seromonitoring (RD)

500.00 / 5 sera

  11.

MPN

200.00

  12.

Plate count

300.00

  13.

CMT reagent

250.00/ 500 ml

  14.

Subclinical mastitis

20.00

  15.

Attending diseases outbreak & investigation

2000.00

  16.

Consultancy during outbreak / Visit

500.00 

Through Lectures, visit at farmers doorsteps, camps, exhibitions, rallies, adopted village, consultancy, Marathi article published in local Newspapers for farmers, Preparing leaflets and Pampltes for farmers ,through toll free services at university level as well as at department etc