Microbiology

The academic staff of department is regular participant in several national and international congress/ conferences. Research work of two boys and one girl student from Denmark was also undertaken and supervised in this department. 

The Department of Veterinary Microbiology was established since inception of the college in the year 1958 with the aim to impart undergraduate education. Initially only under graduate teaching was under taken. The postgraduate program in Veterinary Microbiology was incepted from 1995 and subsequently in Animal Biotechnology from 2007. The Ph.D program in Veterinary Microbiology was started from 2013. Currently the department offers Undergraduate, Postgraduate and Doctoral degree programs. The curriculum prepares students to meet their goals, which include professional careers in academic, clinical and technical field of Microbiology and Animal Biotechnology. So far 45 students have completed PG programs in Veterinary Microbiology and 17 in Animal Biotechnology. Four students have completed their Ph.D. and another two are undergoing Ph.D. program in Veterinary Microbiology.

The department undertakes time-bound research projects, in addition to the teaching and diagnostic services being provided for diagnosis of bacterial, viral and fungal diseases of livestock and poultry.

Heads of the Department:

Dr. S.L.Manjarekar, Dr. S.P.Phadke, Dr. M. S. Dhake, Dr. M. M. Kulkarni and Dr. R. S. Kolhe initially contributed significantly for the development of the department. Later on Dr. S. D. Harne geared up the developmental activities of the department. Subsequently Dr. D. R. Kalorey played a key role in the overall development and upliftment of the department.Recently Dr.V.C. Ingle is superannuated in March 2020 and Dr. Shubhangi Warke (Bhagat)  took the charge of Head of the Department.

Sr. No.

Name of The Head

 

Duration

1.

Dr. S.L.Manjarekar

1958-1964

2.

Dr. S.P.Phadke

1964-1969

3.

Dr. P.R.Dhake

1969-1975

4.

Dr. R. S. Kolhe

1975-1980

5.

Dr. S. D. Harne

1980-1997

6.

Dr. D. R. Kalorey

1997-2014

7.

Dr. V.C.Ingle

2014-2020

8.

Dr. Shubhangi Warke (Bhagat) 

2020-till date

Present status of the Department

A) Staff position (Teaching and Non –teaching)

Veterinary Microbiology:

Position

Name of faculty

Qualification

Professor

I/c Dr. Shubhangi Warke (Bhagat) 

M.V.Sc., Ph.D.

Associate Professor

—

—

Assistant Professor

Dr. Shubhangi Warke (Bhagat) 

M.V.Sc., Ph.D.

Assistant Professor

Dr.P.A. Tembhurne

M.V.Sc., Ph.D.

Sr. Laboratory  Technician

Dr. Jiwan Kesarkar      

M.V.Sc.

 Animal Biotechnology

Position

Name of faculty

Qualification

Assistant Professor

—

—

Senior Research Fellow

Vacant

B)  Infrastructure facilities:

i) Laboratories:

Sr. No.

Name of Laboratory

Purpose

1.

Bacteriology and Mycology Laboratory

For carried out separate  work of Bacteriology and Mycology.

2.

Veterinary Immunology Laboratory

For carried out separate  work of Immunology

3.

Virology Laboratory

For carried out separate  work of Virology

4.

Molecular Diagnosis Laboratory

For carried out molecular biology work

ii) Equipments available for Teaching and Research

Sr. No.

 Instruments available for Research

 Availability

1.

Laminar flow  (Vertical /Horizontal)

 3

2.

Binocular Microscope

 3

3.

Deep fridge ( -200C)

 4

4.

Thermal cycler (PCR machine)

 2

5.

Gel Documentation system

 1

6.

CO2 Incubator

 1

7.

Inverted Microscope

 1

8.

Refrigerated centrifuge

 2

9.

Deep freezer ( -800C)

 1

10.

ELISA reader

 1

11.

Spectrophotometer

 1

12.

Real Time PCR

 1

13.

Gel Electrophoresis apparatus

 2

14.

Lyophilizer

 1

15.

Hybridization oven

 1

Dr. Shubhangi Warke (Bhagat)                   

(MVSc, Ph.D., DBM, NET)                                                            

Assistant Professor & Head

Phone – (O) 0712 2510087,

(Fax) 0712 2510883, (M) 9561214395

Email: shubhnagiwarke@mafsu.in

            shubhangi_warke@rediffmail.com

            shubhangiwarke.sw@gmail.com

Field of specialization : Veterinary Microbiology

 

Teaching

  • *UG, PG & Ph.D. teaching in Veterinary Microbiology
  • * UG teaching in Fishery College
  • *Laboratory manuals prepared for Veterinary Microbiology UG ,PG  & Ph.D.Teaching,

     *Paper setter for UG theory examinations for different Universities, MPPSC, MPSC

     *Participation as External Examiner for  UG,PG & Ph.D. of different Universities.

  • *Guiding students for ICAR,MPSC Entrance and interviews

Research:

MVSc research: STUDIES ON EFFECT OF AQUEOUS HERBAL EXTRACTON GROWTH OF ASPERGILLUS OCHRACEOUS, OCHRATOXIN PRODUCTION AND ITS NEUTRALIZATION

Ph.D. research: Genotypic and Phenotypic Characterization of Listeria monocytogenes from bovines and their environment.

P.G. Students Guided:

Major Guide in Veterinary Microbiology: 06

Member of advisory Committee in Veterinary Microbiology & Animal Biotechnology:15

Member of advisory committee in other disciplines: 12

Ph.D. Student Guided as Major Guide : 01

Member of advisory Committee in Other Discipline :01

 

 List of Year-wise Publications:

Sr.

No.

Authors

Year

Title

Journal

Impact factor

1

Bhojne,G.R.,Dakshinkar, N.P., Kalorey, D.R. and Shubhangi Warke .

2000

Gangrenous mastitis in goats

Intas polivet. . 1 (11) : 262.

  

2

Kurkure,N.V., Kalorey D.R., Shubhangi Warke , Sakhare, P.S. and Bhandarkar A.G.                                

2001

Mortality in young broilers due to Pseudomonas aeruginosa

Indian J. Vet. Res. 10 (1) : 55-57

  

3

Kalorey, D.R., Kurkure, N.V., Sakhare, P.S., Shubhangi Warke and Murtuza Ali.

2001

Effect of ‘Growell’ on performance, organ weight and serum trace element profile in broilers.

Asian Aust. J. Anim. Sci.  14 (5) : 677-679

6.0

4

Kalorey, D.R., Shubhangi Warke , Sakhare, P.S. and Kurkure, N.V.

2002

Etiopathology of gangrenous dermatitis in poultry.

Poultry Fortune. September  : 24-25.

  

5

Bache, S.B., Harne, S.D., Kalorey, D.R., Ingle, V.C., Shubhangi Warke and Sakhare, P.S.       

2002

Microbiological investigation of otitis externa in dogs.

Indian J. Comp. Microbiol. Immunol. Infect. Dis. 23 (1): 95-96

  

6

Kalorey,D.R., Kurkure, N.V., Harne, S.D., Ingle ,V.C., Patil, A.A., Sakhare,  P.S. and  Shubhangi Warke .

2002

Congo-red binding ability of E coli.

Indian J. Comp. Microbiol  Immunol. Infect. Dis. 23 (1): 79-80

  

7

Kalorey, D.R., Kurkure, N.V., Shubhangi Warke, Sakhare, P.S., Ingle, V.C. and Harne, S.D. 

2003

Bacteriological investigation of clinical samples of poultry in Nagpur region

Indian J. Poult. Sci. 38 (2) : 181-183

  

8

Kalorey, D. R; Shubhangi Warke and Sakhare, P.S.(2003) :

2003

Antibacterial activity of   aqueous extract of flowers of Butea frondosa Koen ex roxb (Palas ) –

Indian J. Comp. Microbiol. Immunol. Infect. Dis. 24(2) 209-210.

  

9

Kalorey, D. R;  Dakshinkar, N.P., Kurkure, N.V., Shubhangi Warke Sakhare.,  P.S. and  N.K. Nighot.

2004

Antibacterial activity of a herbal ear drops for pets.-

The Veterinarian. 28:20-23.

  

10

Shubhangi Warke, P.S. Sakhare, A.A.Patil and D.R. Kalorey.

2004

Bacteriological   investigation of canine enteritis. –

Indian J. Vety. Res.13 (2): 50

  

11

Kalorey, D.R., Kurkure, N.V., Ramgaonkar, J.S., Sakhare, P.S., Shubhangi Warke  and N.K. Nighot .

2004

Effect of toxiroak during induced individual and combined Mycotoxicosis in broilers : Growth and haematobiochemical study.

Indian  J.  of Vety. Pathology. 28 (2) : 113-117.

4.6

12

Chousalkar, K.K., Shital Taram, Jyoti Bodade, Prajkta Lonkar, Shubhangi Warke, Kalorey D.R.,Ingle, V.C. and Godbole, S.M.

2004

Serotyping and             antibiogram of E. coli isolate from pigeon.-

Indian J. Comp. Microbiol. Immunol. Infect. Dis.  25 (1) 69-70.

  

13

Kalorey, D. R; Shubhangi Warke , Jyoti Bodade and Chausalkar,K,K.

2004

Characterization of coagulase positive staphylococci isolated from bovine sub-clinical mastitis

Indian J. Comp. Microbiol.Immunol. Infect. Dis.25 (1) 63-64

  

14

Kalorey, D.R., Kurkure,N.V., amgankar,J.S., Sakhre,P.S.,Shubhangi Warke and                                                 Nighot,N.K.

2005

Effect of polyherbal feed supplement “ Growell“ during induced aflatoxicosis, ochratoxicosis and combine mycotoxicosis in broilers

Asian- Aust. J. of Anim.Sci. 18(3) 375-383.

6-0

15

Pathak,M.A., dalvi, G.W., Godbole, S.M., Kalorey, D.R., Shubhangi Warke and Ingle,          V.C.

2005

Isolation of Ecoli from captive Wild animals and their antibiogarm –

Indian Vet. Med. Jour., 29  295-296.

  

16

Kalorey, D. R; Kurkure,N.V., Shubhangi Warke ,Rawool, D.B., Malik, S.V.S. and Barbuddhe,S.B

2006

Isolation of pathogenic Listeria monocytogenes in faeces of wild animals in captivity

Comp. Immunol. Microbiol. Infect. Dis. 29: 295 -300

7.8

17

Kalorey, D. R; Shubhangi Warke and Sakhare., P.S.

2006

In vitro efficacy of                 Newcharm gel against various bacteria and fungi.

Indian Veterinary Medical Journal

  

18

Kalorey, D.R., Chausalkar K.K., Shubhangi Warke & Dhote S.W.

2006

Study on efficacy of mastilep in the treatment of subclinical mastitis.

Indian Veterinary Medical Journal.

  

19

Shubhangi Warke., Kalorey,D.R., Kurkure,N.V.& Barbuddhe,S.B.

2007

Comparative efficacy of culture media for isolation of Listeria spp. From Bovine milk samples.

RVJI 3: 78-80.

  

20

Shubhangi Warke., Kalorey, D.R., Kurkure, N.V.& Barbuddhe, S.B.

2007

Prevalance and Genotypic Characterization of Listeria monocytogenes of bovine intramammary origin.

RVJI 3 : 113-117

  

21

Shubhangi Warke, D.R. Kalorey and N.V. Kurkure .

2007

In vitro antiochratoxigenic and ochratoxin A neutralization potential of aqueous herbal extracts.

Indin J. Comp. Microbiol.Immunol.Infec.Dis. 27:26-29.

  

22

Kalorey, D. R; Shubhangi Warke. Kurkure, N.V., & Barbuddhe,S.B.

2007

Seropositivity against Listeriolysin in Cattle of Vidharbha Region

J. Vety. Pub. Hlth.4:117-119.

  

23

D.. R. Kalorey, N. V. Kurkure, S. R. Warke and S. B. Barbuddhe.

2007

Evaluation of Indirect and Avidin–Biotin Enzyme Linked Immunosorbent Assays for Detection of Anti-Listeriolysin O Antibodies in Bovine Milk Samples.

Zoonoses and Public Health 54:301-306.

  

24

Sakhare, P.S., Harne S.D., Kalorey D.R., S.R. Warke, Bhandarkar A.G. and             Kurkure N.V.(2007):

2007

Effect of Toxiroak polyherbal feed supplement during induced aflatoxicosis, ochratoxicosis and combined mycotoxicoses in broilers.

Vet. Arhiv. 77: 129-146.

  

25

Jyoti Bodade., Prajecta Loankar., Kalorey,D.R., Ingle,V.C., Kurkure, N.V., Shubhangi Warke and Godbole.,S.M.

2007

Serotyping and antibiogram of bacterial isolates from Poultry Samples

Indian J. Poult.Sci. 42: 117-119.

  

26

Kalorey,D. R. S. R. Warke, N. V. Kurkure, D.B. Rawool, and S.B. Barbuddhe  

2008

Listeria species in bovine raw milk: a large survey of Central India.

Food Control 19:109-112

7.7

27

Kalorey,D. R. Uma Tumlam and Shubhangi Warke

2009

Kamdhenu ark: curing of plasmid from mastitogen and synergistic action with antibiotics.

J. immunology and immunopathology .11(1) :21-25.

3.1

28

Kalorey,D. R. Uma Tumlam and Shubhangi  Warke 

2009

Modified kamdhenu ark: curing of plasmid from mastitogen and synergistic action with antibiotics.

J. immunology and immunopathology .11(1) :26-29.

3.1

29

Suryawanshi,V.P., Kalorey,D.R. S.R. Kathwate, Sonal Ingle and Shubhangi Warke 

2010

Polyherbal tooth powders as an antimicrobial agent.

Indian J. of Field Veterinarians .(5)53-55.

  

30

Husain,A.A. Kashyap.R.S., Kalorey,D.R, Shubhangi Warke, Purohit, H.J., Taori,G.M. and Daginawala,H.F. 

2010

Effect of repeat dose of BCG vaccination on humoral response in mice model.

J. Expt. Bioogy (49) 7-10

7.8

31

Sonal Ingle, Durge, S.M., Jadhave,S. R.Kathwate,  A.D. Sonegaonkar , Shubhangi Warke and Kalorey,D.R.

2010

Congo –red binding ability and antibiogram of Escherichia coli isolates from domesticated pigs

Indin J. Comp. Microbiol.Immunol.Infec.Dis. 31 (1&2)  65-66

  

32

 Husain AA, SR Warke, Kalorey DR, Taori GM, daginawala HF, Kashyap RS.

2015

 Comparative evaluation of booster efficacies of BCG, Ag 85B, and Ag85 B peptides based vaccines to boost BCG induced immunity in BALB/c mice. A pilot study.

Clinical and Experimental Vaccine Research .4:83-87

9.28

33

Husain AA, Daginawala HF,SR Warke,Kalorey DR, Kurkure NV, Taori GM, Kashyap RS.

 2015

 Investigation of immune biomarkers using subcutaneous model of M. tuberculosis infection in BALB /c mice

Immune Network .15 (2) 83-90.

0.4

34

Aliabbas A. Husain,Hatim F. Daginawala• Shubhangi R. Warke,Dewanand R. Kalorey• Nitin V. Kurkure,Amit R. Nayak• Hemant J. Purohit Girdhar M. Taori• Rajpal S. Kashyap  (2015)

2015

Heterologous Prime Boost Regimes with N-terminal Peptidesof Ag85B Induces Better Protection than Ag85B and BCGin Murine Model of Tuberculosis

Int J Pept Res Ther DOI 10.1007/s10989-015-9490-8  Published online , 16 th September

6.19

35

Prasad D.,S.R. Warke Bobade S,Kalorey D.R.

2015

DNA fingerprinting andRAPD PCR analysis of S. aureus strains isolated from bovine origin.

Indin J. Comp. Microbiol.Immunol.Infec.Dis. 36 (2):80-86.

  

36

Sumedha Bobade, Shubhangi Warke, Devanand Kalorey

2016

Virulence Gene Profiling and Serotyping of Listeria Monocytogenes from Infertility Cases of Women.

International Journal of Health Sciences & Research (www.ijhsr.org) IJHSR.Vol.6; Issue: 1; January 2016. 440-449

Not in NASS

37

Sumedha Bobade, D.R. Kalorey, and Shubhangi Warke.

2016

Biosensor Devices: A review on their biological applications..

Biosci. Biotech. Res. Comm9(1): 132-137 

3.48

38

Uma Singh, Shubhangi Warke, Dewanand Kalorey , Sumedha Bobade

2016

Characterization of Listeria monocytogenes from various sources by Random amplified polymorphic DNA(RAPD) and serotyping.

Indian J. Comp. Microbiol. Immunol. Infect. Dis. Vol. 37 No. 1 (Jan-June), 2016: 37-42

3.16

39

Trivenee Thakre, Shubhangi Warke, Sumedha Bobade and Kalorey D.R.(2017)…  NASS Imapact fator 6.04. cited by 1

2017

Characterization of E coli pathotypes of bovine and livestock farm environment origin

Indian Journal of Anim. Res.  http://dx.doi.org/10.18805/ijar.v0i0f.3784  51 (6) 1125-1129

6.04

40

S.R.Warke,V.C.Ingle, N.V.Kurkure,P.A.Tembhurne, S.P. Chaudhari and S.B. Barbuddhe

2017

Biofilm Formation and Associated Genes in Listeria monocytogenes. The Indian Journal of Veterinary Science and Biotechnology

IJVSBT 12( 3) 07-12

4.45

41

Barbuddhe,S.B.,Doijad,S.P.,Goesmann,A.,Hilker,R.,Poharkar,K.V.,Rawool,D.B.,Kurkure,N.V.,Kalorey,D.R. ,Malik,S.S., Shakuntala,I.,Chaudhari S.,Waskar,V.,D’Costa,D.D.,Kolhe R., Arora,R,Roy,Ashish.,Raorane,A., Kale,S.,Pathak,A.,Negi,M.,Kaur,S., Waghmare,S.R. Warke, Shoukat,S., Belgode H., Poojary,A., Madhavprasad,C., Nagappa,K., Das S., Zende,R.,Garg,S.,Bhosle,S., Radriguez,S.,Paturkar A., Fritzenwanker M., Ghosh,H., hain,T.,and Chakraborty,T.

2017

Presence of a widely disseminated Listeria monocytogenes serotype 4b clone in India.

Emerging Microbes & Infections .5,e55;doi:10.1038/emi.2016.55 published online 8th june,2016

11.61

42

Shubhangi Warke, Sumedha Bobade, D. R. Kalorey and V. C. Ingle

2017

Inflammasomes as an activated molecular platform for engagement of innate immune defenses.

Int.J.Curr.Microbiol.App.Sci 6(6) 1194-1200 https://doi.org/10.20546/ijcrm

5.38

43

Shubhangi Warke, Sumedha Bobade and Vijay Ingle

2017

Isolation and molecular

Characterization of ETEC and NTEC Escherichia coli from cattle farm with reference to

virulence marker gene.”

International Journal of Livestock Research.(IJLR). 7 (10) 2017.  DOI 10.5455/ijlr.20170801052904

5.36

44

Sharmita Doley, V. C. Ingle1, P. A.Tembhurne, S. R. Warke, Prasanna Pati and Nekibuddin Ahmed2

2018

Molecular characterization of Staphylococcus spp. Isolated from respiratory tract of apparently healthy and clinically sick sheep and goat in Nagpur

., India. Indian journal of Animal research.52(6) 907-910

6.2

45

Shubhangi Warke, Sumedha Bobade,Tembhurne, P.A. and Ingle V.C

2019

Paleovirology: Blessing or Curse of Ancient Viruses

Agricultural Review.40 (4) 329-333.

4.37

46

Dorlikar P.R., S.R Warke, UM Tumlam,V.C. Ingle

2019

Comparative Efficacy of Canine Parvovirus-2 Infection Diagnostic Tests Commonly Prevailing in India

The Indian Journal of Veterinary Sciences and Biotechnology14 (4 ) 51-52.

4.45

47

Thorat, S.R., Rawool, D.B., Sonkusale, P.M. Warke S.R., Choudhari , S.P. and Kurkure , N.V.

2019

Virulence Profiling of Listeria monocytogenes Isolated from Different Sources

Int.J.Curr.Microbiol.App.Sci  8(5): 2010-2017

5.3

48

Dorlikar.P.R, S.R.Warke, U.M.Tumlam and V.C.Ingle

2019

Isolation and Identification of Canine parvovirus Infection from around Nagpur region

Journal of Entomology and Zoology Studies” 7 (4) 216-219.

5.53

49

Tumalm Uma, Ingle, V.C. and Warke S. R.

2019

Molecular characterization and phylogenetic analysis of rotavirus of human infants, calves and piglets

Journal of Entomology and Zoology Studies”7 (4) 956-960.

5.53

50

Shubhangi Warke , Ingle,V.C. and Tumlam,Uma (2019). NAAS rating.5.53

2019

Isolation  and molecular characterization of Listeria monocytogenes in bovines and their environment

Journal of Entomology and Zoology Studies”7 (6) 339-348

5.53

51

Shubhangi Warke, and Ingle,V.C

2019

Incidence of Listeria monocytogenes in bovine environment with respect to phenotypic and genotypic characterization.A review

International J. of Current Microbiology & applied Sciences. 8(11) 1-28

5.53

52

Sruthy S, Tumlam UM,SR  Warke , VC Ingle, Bhojane GR and Hedau M.S .

2019

VP6 gene based RT-PCR for detection of rotavirus associated with diarrhea in bovine calves

Journal of Entomology and Zoology Studies; 7(6): 373-376,

5.53

53

Sunny Magar , Shubhangi Warke , Ingle ,V.C. and Tumlam Uma

2020

Molecular typing of Canine Parvovirus -2 occuring in Nagpur by Multiplex PCR.

Journal of Entomology and Zoology Studies”8(1) 91-95.

5.53

54

Kaul , Viraj., Shubhangi Warke & Tumlam Uma

2020

Anti-microbial peptides: new weapon against bacteria”

Int.J.Curr.Microbiol.App.Sci. (2020).9(6):2313-2319

5.38

55

Khadse Monali and Shubhangi Warke 

2021

Mud Power : How bacteria can turnWaste in to Electricity.

wjahr.5 (2) XX-XX. SJIF

5.46

56

Sreelaxshmi Nair & Shubhangi Warke 

2021

Radioactive Fungi : A New Horizon of Fungi

World Journal of Advance Health Research

Wjahr 5(6) 232-235 SJIF

5.46

57

Shubhangi Warke

2022

Recent advances for Cancer & Infectious Disease Diagnostic : Sequencing

Wjahr 6(2) 53-58

5.46

58

Shubhangi Warke

 

2022

Molecular characterization of L. monocytogenes isolated from milk and milk products

The Indian J. of Vety. Science & 18 (3) 108-111. Biotechnology.

5.58

59

Shubhangi Warke

2022

CRISPR -based tools : Alternative methods for diagnosis of diseases

The Pharma Innovation SP-11(9): 1891-1896.

5.23

60

Vrushali Dhas and Shubhangi Warke

2022

Synthetic biology : Application in disease diagnosis.

The Pharma Innovation SP-11(12): 119-123.

5.23

61

Khadse M.B., Warke Shubhangi and Kolangath, Sujit

2023

Molecular detection and Phylogenetic analysis of Canine parvovirus -2 in dogs

The Indian J. of Vety. Science & Biotechnology. 19 (2) 54-57.

5.58

62

Warke Shubhangi., Ingle V.C. and Kurkure N.V.

2023

PFGE analysis of Listeria monocytogenes isolates from Milk, Bovine clinical and Bovine Environment origin from Nagpur.

The Indian J. of Vety. Science & Biotechnology19 (2) 78-82..

5.58

63

Shubhangi Warke and Sumedha Bobade 

2023

Serotyping ,RAPD PCR , Antibiotic Susceptibility Profiling of Listeria spp. Isolated from Raw Chicken Meat.

International Journal of Research and Review 10(5) 528-537

Not in NASS

64

Sonkusale,P.M. Warke S.R. Chaitanya,S. and Sukirti Sharma .

2023

Pathological Studies and Molecular detection of Salmonellosis in desi Chickens of Nagpur Region.

International Journal of Research and Review 10(5) 523-527

Not in NASS

65

Warke S.R., Sonkusale,P.M. and Boabde , S.S.

2023

Prevalence study of Biofilm marker ica D gene among Coagulase Positive Staphylococcus of Mastitic Origin

International Journal of Current Science Research and Review. 6 (6) 3140-3147

6.78

66

Sonkusale,P.M. Warke S.R. Chaitanya,S. and Sukirti Sharma, Kamble M.V. and Khati B.M. (2023).

2023

Pathology and Molecular detection of Avian Colibacilossis in Commercial Chickens of Nagpur Region

International Journal of Current Science Research and Review. 6 (5) 2937-2940.

6.78

67

SR Badhe, KS Rathod, SP Chaudhari, AP Dhok, SR Warke, Anupama Kumar and BL Mundhe

2023

Paneer preservation puzzle: Unraveling the link between microbial spoilage and chemical clues in refrigerated storage..

The Pharma Innovation Journal 2023; SP-12(10): 991-996

5.23

68

Shubhangi Warke and Sunny Magar.

2023

Molecular Characterization of E coli isolated from faecal samples of Pups affected with Canine Parvoviral Entritis.

The Indian J. of Vety. Science & Biotechnology. 20 (1) 22-26.

5.58

69

Shubhnagi Warke and Sumedha Bobade  .

2023

Phenotypic and Molecular Characterization of Listeria Spp. from Raw Chicken Meat

The Indian J. of Vety. Science & Biotechnology. 19 (2) 54-57.

5.58

70

Shubhangi Warke,Gayatri Chavan,Mehak Tikoo and Sujata Bhatkar .

 

2023

Molecular identification of Malassezia pachydermatis isolated from dermatitis cases in dogs.

The Pharma Innovation Journal SP-12 (11): 2142-2144

5.23

71

.VirajKoul,S.R.Warke,U.M.Tumlam,Mehak Tikoo and S.S. Bobade .

2024

Molecular Charcterization and Phylogenetic Relationship of VP6 Gene of Rotavirus among Canine,Porcine and Human Infants.

Indian Journal of Animal Research  doi.: 10.18805/IJAR B-5238

6.50

72

SR Badhe, KS Rathod, SP Chaudhari, AP Dhok, SR Warke, Anupama Kumar, BL Mundhe and DV Patil .

2024

Assessment of paneer quality during refrigerated storage

International Journal of Agriculture Extension and Social Development .7 (2) 93-99

5.04

73

SR Badhe, KS Rathod, SP Chaudhari, AP Dhok, SR Warke, Anupama Kumar, BL Mundhe .

2024

Chemical based smart label to communicate real time freshness of packed paneer.

Food Research International . Accepted in Jan22,2024

14.10

 

Research Projects:

Completed Research projects:

Title of Project

Funding agency

   Amount in Lakhs

Date of Sanction and Duration

Genetic Diversiy of Listeria monocytogenes with different virulence potential

( Principle Investigator)

DST

10.27200/-

2010 -2013

Virulence profiling of Listeria monocytogenes isolated from food-, veterinary and clinical sources

 (Co- Investigator)

ICMR(Indo German)

10.00/-

2011-2013

Translational research on Molecular epidemiology of L.monocytogenes

 (Co- Investigator)

CEIB

(DBT)

50.00/-

2012( 5 years)

DBT Network Project on Brucellosis

 (Co- Investigator)

DBT

1,14,699/-

2012 (5 years )

Star College Scheme for Strengthening of Science & Biotechnology Education and Training at Undergraduate Level

(Co-Coordinator)

DBT

19.00/-

2019-2022 (3 years)

 

Completed Sponsored Project

Title of Project

Funding agency

 Amount  in Lakhs

Date of  Sanction and Duration

To study the safety & immunogenicity of Brucella abortus RB51, Vaccine in cattle

(Co-PI)

IIL

48,750/-

      1st to 31st November,2010

Evaluation of  mastodon in bovine mastitis

(CO-PI)

Cattle Remedies India Ltd.

80,040/-

 January, 2012 to August ,2012

An open label multicentric clinical trial to study safety and immunogenicity of Tetanus toxoid vaccine in animals

(PI)

Indian Immunologicals

2.29200/-

March 2019- Sept 2019

Ongoing Project

Efficacy evaluation of some herbal formulations for control of Mycoplasmosis in commercial broilers

(CO-PI)

Ayurvet Ltd., HP., India

3,00,000/-

February, 2023

.

PUBLICATIONS:

1) Scientific research paper published in International Journals   : 11  

2) Scientific research paper published in National Journals           :61

3) Research paper presented at International Seminar                   :09

4) : Research paper presented at National  Seminar                       :118

5) Popular article                                                                            : 62

6) Books                                                                                          : 03

7) Book Chapters                                                                           : English: 05 Marathi :04

 

ACHIEVEMENTS:

*    Identified herbs with antiochratoxic & OA neutralizing activity

*   Developed Milk based  Dot ELISA for detection of   L. monocytogenes

*    Developed Milk based Indirect ELISA for detection of   L. monocytogenes

*    Developed Milk based  AB ELISA for detection of   L. monocytogenes

*    Developed Serum based  Indirect  ELISA for detection of   L. monocytogenes

*   Isolation & characterizations of Listeria from wild life

*   Antibacterial activity of herbal extracts

*   Developed multiplex PCR for detection of virulence marker genes of Listeria spp.

*  Identify Disinfectant  to reduced contamination of L. monocytogenes at bovine farm.

* Developed Multiplex PCR for detection of antigen of ParvoVirus

* Standardized qPCR for detection of L.monocytogenes from Bovine environment

*  Standardized multiplex PCR and Nested PCR for detection of Canine parvovirus infection

* Developed qPCR for detection of Canine parvovirus infection

* Developed LAMP test for detection of Canine parvovirus infection

* Standardized PCR for detection of VP6,VP4 & VP7 gene of Rotavirus in Human infants ,  Porcine and Canines

*  Gene Sequence  submitted  to gene bank (NCBI) : 60

 Research  Recommendation:

 

Sr.No.

Project No.

Research Workers

Recommendation

1

NVC/micro/22/2018-2019

Dr.Shubhangi Warke

Dr.V.C.Ingle

Use disinfectant Didocyldimethyl Ammonium Chloride(DAC) to reduced contamination of Listeria monocytogenes at livestock farm

2

NVC/Micro/09/2019-2020

Dr.Sunny Magar

Dr.Shubhangi Warke

Use multiplex PCR using VP2 gene to detect antigenic types of Canine parvovirus (CPV)  from faecal swab of canines

3

NVC/Micro/07/2023-2024

Dr.Vrushali Dhas

Dr.Shubhnagi Warke

The Standardized LAMP assay is recommended to use for the direct detection of canine parvovirus in fecal samples
       

 

 

AWARDS & HONORS:

 

1.Fellowship :Dr. B.V. Rao WPSA (IB) WPC, 1996. Research Grants 2000 for Post Graduate       Research Program.

  1. Ayurvet Best Research Article – IX Annual Conference of IAAVR , 2002.
  1. Best Poster award – IX Annual Conference of IAAVR, 2002
  1. Young Scientist Award -2009 for outstanding service in the field of Veterinary Science
  1. World Environment Educational award -2012
  1. Mother Teresa Sadbhawana Award-2015

7.Certificae of Appreciation -2016 International Womans Day

8.Appreciation Certificate  for participated to organized XXXth annual Convention& National Symposium of IAVMI at Nagpur from 10-12 th February ,2017.

9.Letter of Appreciation in recognition as  a Member of Convocation Cell of eighth convocation of Maharashtra Animal & Fishery Sciences University held during 9 th March ,2017

10 Best poster award– First best Poster Presentation Award during XV Biennial Conference and National Symposium of IAWV at Hyderabad  during 21-22 ,November, 2017.

11.First Prize for oral Presentation at National Symposium  at Akola during 26th -27th November,2019.

12 Second Prize for poster presentation at  National Symposium at Akola during 26th -27th November,2019.

 

13 Letter of Appreciation:  for worked as Member in judging Committee for Elocution & Drawing Competition held on the occasion of “World Animal Day” on 4th October, 2019,  at Nagpur Veterinary College, Nagpur .

  1. Appreciation Certificate for outstanding contribution and dedicated efforts for successful organization of National conference & XVI Annual Symposium of IAVPHS on 26 and 27 th February,2019 at NVc., Nagpur

 

15.Certificate of Honour for Best Paper Presentation award in Canine Microbiology & Immunology Session 2019   In XVII National Congress on Canine Practice & Natiional Symposium  at Jabalpur(M.P.)  during 21-23 Jan 2020.

16 Second Poster presentation  (2020) In Online International conference: WILDCON 2020  14th Annual Convention of Association of Indian Zoo & wildlife Veterinarians organized by WRTC, Gorewada, Nagpur . during 18-20 December, 2020.

  1. Mother Teresa Sadbhawana Best Teacher Award with Medal 2020 .on occasion of National Seminar “ Individual Achievements and National Development Tuesday,26th January,2021 at New Delhi.

18.Letter of Appreciation in recognition as  duties performed in  Tenth convocation of Maharashtra Animal & Fishery Sciences University held during 10 th November ,2021.

  1. Certificate of Appreciation from Guru Nanak College,Chennai Internal quality Assurance Cell.E -Quiz on Revised NAACAssessment and Accreditation Procedure on 28th June,2021.
  2. Global Eminent Scientist Award (2022) for outstanding Service in Research presented on 25/7/2022 at Thirunindravur (TN) by VIJ trust.
  3. Second Prize of Best Clinical Case Paper Presentation Award (2023) 6th National online Clinical case Conference.during 25-27 th April , 2023 organized by Department of Veterinary Surgery & Radiology .P.G.I.V.A,S., Akola (MS).
  4. IDEATHON -2023 award : Second Prize in the theme Animal Health IDEATHON ,2023 conductrd under the aegis of Indian Society of Veterinary Immunology and Biotechnology (ISVIB) at College of Animal Biotechnology ,Guru Angad DevVeterinaryand Animal Sciences University ,Ludhiana.

23.Woman Scientist Award : for outstanding contribution in the field of Veterinary Microbiology on the occasion of 3 days International Conference (ICCGFLLES-2023) Jointly organized by NAU,Navsari,Gujrat and NADCL,Baramulla,Ut of J & K in Collaboration with ICAR-NAHEP,Centre for Advanced Agricultural Science & Technology (CAAST),NAU,Navsari,Held at main campus, Navsari Agricultural University Navsari,Gujrat during 28-30 December,2023.

24.Excellence in Research Award: for outstanding contribution in the field of Veterinary Microbiology on the occasion of 3 days International Conference (ICCGFLLES-2023) Jointly organized by NAU,Navsari,Gujrat and NADCL,Baramulla,Ut of J & K in Collaboration with ICAR-NAHEP,Centre for Advanced Agricultural Science & Technology (CAAST),NAU,Navsari,Held at main campus, Navsari Agricultural University Navsari,Gujrat during 28-30 December,2023.

  1. For outstanding Individual Accomplishment & Distinguished Veterinary Services to the Nation on the occasion of Republic Day of India, 26th Jan.2024. By Pashuprahari

Organization of Event:

 

  • National Symposium on “Challenges in animal health for higher productivity and Income to farmers” and XXX Annual Convention of IAVMI , Nagpur February 10-12,2017.-Joint Seretary
  • International Webinar on “COVID-19 Virulence in Aged Patients Might Be Impacted by the Host Cellular MicroRNAs”  on dated 6th August,2020.- Organising Secretory
  • International Webinar on “Epigenetics therapy in Cancer” on Dt. 7th August , 2020 – Organising Secretory
  • National Webinar on “ Importance of Biosafety In the scenario of infectious Diseases outbreak” on dated 10th November ,2020– Organizing Secretory
  • National Webinar to celebrate World Antimicrobial Awareness week on “ Antimicrobial Resistance : A complex global issue” on dated 24th November,2021 Organizing Secretory
  • National webinar on “Clean milk production: aneed of the day” on dated 3rd December, 2021. Organizing Secretory
  • Organised Lecture on Clean Milk Production & Demonstration on Dectection of Mastitis by CM T on dated 3rd December, 2021 at Mohapa, Tel.Kalmeshwar, District Nagpur
  • Free Antirabies Vaccination Camp to celebrate World Rabies Day on 27th, 2022 at Pratap nagar., Nagpur
  • National Webinar to celebrate World Rabies Day on “An insight into Rabies : control & Strategies” on 28th ,Sept., 2022
  • Eassay Compitition for students to celebrate World Antimicrobial awareness week on 24 th November,2022
  • One day Workshop ,Awareness & Woman empowerment to celebrate clean milk production day on 2nd December, 2022
  • Free Antirabies Vaccination and Awareness Camp on 27th September,2023 at Kalmeshwar, District ,Nagpur to celebrate World Rabies Day
  • National Webinar to celebrate World Antimicrobial Awareness week on “Antimicrobial Resistance : A One Health Perspective” on dated 23th November,2023 Organizing Secretory
  • Essay Compitation : for students to celebrate World Antimicrobial awareness week on 24 th November,2023
  • Trainings : 04

 

EXTENSION:

 Extention and Field Outreach Activities : Technical guidance to farmers and Field Veterinarians through camps, lectures, trainings, radio talk , T.V. talks, Lectures to Pashusakhi  & visit at adopted villages etc.

Dissemination of technical knowledge through article publications in local news paper , leaflets & pamphlets

MEMBERSHIP:

Membership of Scientific organizations (11)

  • Life Member of Indian association for the Advancement of Veterinary Research (IAAVR)
  • Life member of Indian association of Veterinary Public Health Specialist (IAVPH),
  • Society for Immunology &Serology
  • Indian Veterinary Community
  • Life member of  National Association  for welfare of Animal Research (NAWAR)
  • Life Member of Society for Listeria
  • Life Member of Indian Association for Woman’s  Veterinarians’  (IAWV)
  • Life Member of  Indian Society of Canine  Practitioner (ISACP)
  • . Vaikalpic pashu chikissa vidhanan

11) Indian Society of Veterinary Immunology & Biotechnology (ISVIB)

                                                                                       

Name:

Dr Prabhakar A. Tembhurne

Qualification

MVSc, PhD, PDF(USA))

Designation:

Assistant  Professor

Field of specialization:

Veterinary Virology

Field of Interest

small RNA biology (miRNA),  Molecular diagnostics &  epidemiology  of animal pathogens, Host-pathogen interaction

Research:

 

List of Year wise Publications

  1. Sudhakar Awandkar, Prabhakar A. Tembhurne, Nitin V. Kurkure, Sandeep P. Chaudhari, Sachin W. Bonde & Vijay C. Ingle (2024) .Effect of silver nanoparticles on antibody response against recombinant VP2 protein of Infectious bursal disease virus .Indian Journal of Experimental Biology Vol. 62, January 2024, pp. 7-16 DOI: 10.56042/ijeb.v62i01.1609.
  2. Juhi Jain1, Yash Chaudhary, Sharad Kumar Gaur, Prabhakar Tembhurne , Shanmugam Chandra Sekar, Muthuchelvan Dhanavelu, Sharvan Sehrawat and Rajeev Kaul (2023). Peste des petits ruminants virus non-structural V and C proteins interact with the NF-κB p65 subunit and modulate pro-inflammatory cytokine gene induction, Journal of General Virology  2023; Volume 104 , Issue 10 https://doi.org/10.1099/jgv.0.00190714
  3. Chaudhary, Y.; Jain, J.; Gaur, S.K.; Tembhurne, P.; Chandrasekar, S.; Dhanavelu, M.; Sehrawat, S.; Kaul, R. (2023.)Nucleocapsid Protein (N) of Peste des petits ruminants Virus (PPRV) Interacts with Cellular Phosphatidylinositol-3-Kinase (PI3K) Complex-I and Induces Autophagy..Viruses2023,15,1805. https:// doi.org/10.3390/v15091805 ,82
  4. Aniket Thakare, Prabhakar A Tembhurne, Jivan Kesharakar, Deepali Gharat, Gayatri Chavan and Sudhakar Awandkar (2023). Expression of bluetongue virus rVP7 protein in prokaryotic expression system and evaluation of crude lysate for Dot-ELISA.The Pharma Innovation Journal; SP-12(11): 2250-2256  23 ( NAAS rating 2023 )
  5. Payghan PS, Bhojne GR, Dhoot VM, Panchbhai CG, Kolte SW, Tembhurne PA and Shrideeksha . Therapeutic management and Haemato-biochemical alterations in diarrhoeic dogs affected with ancylostomosis. The Pharma Innovation Journal (2023); 12(5): 4669-4672
  6. M. Kolangath, S.V. Upadhye, V.M. Dhoot, M.D. Pawshe, A.S. Shalini, P.A. Tembhurne, S.W. Kolte (Molecular Evidence of Hepatozoon felis Infection in Wild Captured Royal Bengal Tiger Cub (Panthera tigris tigris). Indian Journal of Animal Research ( 2022) B-4674 :1-6 NAAS rating 2022- 6.44
  7. Sharma Y, Sarkar R, Jain A, Singh S, Shekhar C, Shanmugam C, Dhanavelu M, Tembhurne P, Kaul R, Sehrawat SA Mouse Model of PPRV Infection for Elucidating Protective and Pathological Roles of Immune Cells. Front Immunol.Apr. 2021; 12:630307.
  8. Sipra Panda, Ruchi Bhate, Anuradha Nemade, Sneha Thorat, Mayuri Chelkar, Monali Bhagwan, P. M. Sonkusale, A. Tembhurne and N.V. Kurkure. Pathological and molecular investigation of Peste des Petits Rumi- nants outbreak in goats of Nagpur district, India.Indian J. Vet. Pathol., 2021, 45(1) : 66-68
  9. Preeti P. Bramhapurkar, Prabhakar A.Tembhurne, S. Chandra Sekar, D. Muthucheven, Sharvan Sehrawat, Prashant Tarale,Vijay.C. Ingle and Rajeev Kaul. (2020) Circulating MicroRNA-21-3p: A potential Biomarker for Peste- des-petits ruminants virus in naturally infected goats. J.Curr.Microbiol.App.Sci(2020) 9(8): 341-351
  10. J. Ade,  V.C.  Ingle,  P.A.  Tembhurne,  U.M.  Tumlam,  A.S.  Kadam,  G.R.  Bhojne,  and  P.M.  Sonkusale.Incidence  and  Molecular  Characterization  of  Rotaviruses  of  Cattle  and  Buffalo  Calves  inAmravati  Region,Indian J. Vet Sci. Biotech(2019) Vol. 14 No. 320
  11. Pragati Bhoyar, Prabhakar Tembhurne*, Ruchi Bhate, Prashant Tarale, Anuradha Nemade Anand Kadam, Jivan Kesharkar and V.C. Ingle Molecular Detection of Peste des-petits Ruminant Virus (PPRV) by RT-PCR J.Curr.Microbiol.App.Sci (2019) 8(11): 2594-2601
  1. S. Kadam , P. A. Tembhurne*, S. W. Bonde , S. P. Chaudhary , N. V. Kurkure and V. C. Ingle (2019) Molecular Detection of Group A Rotaviruses from the Bhandara and Chandrapur District of Maharashtra State, India (2019)   Int.J.Curr.Microbiol.App.Sci2019) 8(9): 2555- 2564.
  1. Warke, Shubhangi; Tembhurne, Prabhakar; Bobade, Sumedha; Ingle, V. C.Paleovirology: Blessing or Curse of Ancient Viruses: Agricultural Reviews Oct-Dec2019, Vol. 40 Issue 4, p329-333. 5p
  1. UM Tumlam, VC Ingle, PA Tembhurne, NV Kurkure and SP Chaudhari.Detection of rotavirus associated with diarrhea in bovine calves by RNA-PAGE and RT-PCR. (2019) Journal of Entomology and Zoology Studies( 2019); 7(4): 961-964
  1. S. Kadam , P. A. Tembhurne and V. C. Ingle. Bacterial profiling from the bovine  calves diarrhoea and its antibiotic sensitivity pattern around Chandrapur district in Maharashtra .Internatonal journal of Science , Environment and technology, (2018) Vol.7,No. 6, 2019-25
  1. Awandkar SP, Tembhurne PA, Kesharkar JA, Kurkure NV, Chaudhari SP, Bonde SW, Ingle VC . Identification and characterization of a novel infectious bursal disease virus from outbreaks in Maharashtra Province of India, Veterinary World(2018), 11(10): 1516-1525.
  1. M.Tumlam, V.C.Ingle, P.A.Tembhurne, N.V.Kurkure, S.P.Chaudhari, S.D.Chitambar and S. Bhoyar. Detection of VP6 gene of Rotavirus in Feces of Diarrhoeic calves, kids, lambs, piglets,pups and human infants by Reverse Transcriptase–Polymerase Chain Reaction The Indian Journal of Veterinary Sciences & Biotechnology (2018) Volume 13, Issue 4, 12-16

 

  1. Pannerselvam M. Kumar, Prabhakar A. Tembhurne, Paramasivam Raja, Vijay C. Ingle, and Devanand R. Kalorey. Knockdown of gga-miR-142-3p in developing chicken embryo: expression profile of XPO1 Arhiv (2017) 87(1) 67-76.
  2. Raja Paramasivam, Prabhakar Tembhurne, Maneshkumar Pannerselvam, Vijay Ingle, Nitin Kurkure and Dewananad Kalorey. Evaluation of function of RICTOR gene in gga-miR-142-3p knock downed developing chicken embryo Indian J. Anim. Res., 51 (5) (2017) : 807-81
  3. Tembhurne P.A Lead paper : miRNAs-142- 3p: A comprehensive regulator of cellular processes; VIBCON , 2017
  4. Tembhurne P.A., Raja P., Manesh Kumar P., Sanjay Gohain., Shabanam Kumari and Awandkar S.P & V.C Ingle. Lead paper : miRNAs: The Fine Tuner of the Life, National Symopsium & Annual Convention of IAVMI, 10-12 Feb.(2017), Compendium ,( 2017) PP 20-22
  5. R.Warke,V.C.Ingle, N.V.Kurkure, P.A.Tembhurne, S.P. Chaudhari and S.B. Barbuddhe. Biofilm Formation and Associated Genes in Listeria monocytogenes. The Indian Journal of Veterinary Science and Biotechnology. (2017),. 12(3) 7-12
  6. Pannerselvam Manesh Kumar, Paramasivam Raja, Prabhakar Abhimanji Tembhurne, Sanjay Gohain and Vijay Chokharam Ingle. Differential Expression Profile of TGFβ2 in Immune and Visceral Organs of ggamiR-142-3p Knockdown Chicken Embryo. Journal of Animal Research: (2016) 6 No.6 977-982.
  7. Raja Paramasivam , Prabhakar Tembhurne and  Manesh Kumar Pannerselvam. Role  of  gga – mir – 142 – 3p  in  cell  structural  integrity in  developing chicken  J. Env. Bio-Sci., (2016): Vol. 30 (1):173-176
  8. Ghorai S, Ghorai N, Dutta L, Bera A, Ghorui S, Kinhekar AS, Ingle VC, Sonkusale P, Awandkar SP, Tembhurne PA, Kumar V, Ravikumar RK and Kumar V. Protective and Immuno-Modulatory Effect of Low Cost Locally Available Technology from West Bengal, India under IndigenousKnowledge Research System [IKRS] J. Immunol. Immunopathol. (2016) 18(1): 19-23
  9. Ravikumar  K.,  Kinhekar  Amol S.,  Ingle,   V.C. ,Sonkusale  Prashant,  Awandkar  Sudhakar P., Tembhurne  Prabhakar A., Kumar Vipin. Effect of Heat Stress on Haematological and Immunological Parameters in Broiler Chicken.The Indian Journal of Veterinary Sciences and Biotechnology (2016 ),11(3) : 40-42
  10. Raja, P., Manesh Kumar, P., Tembhurne, P. A., Ingle, V. C.  And Kalorey, D. R. Real time expression analysis of RAC1 gene in gga-mir-142-3p knockdowned developing chicken embryo. Journal of Cell and Tissue Research, (2015) Vol. 15(3) 5317-5321
  11. Manesh Kumar, P., Raja, P and Tembhurne, P. A. Efficacy of  intravenous  inoculation  of  evans  blue  dye  (EBD)  to Differentiate  dead  and  live  chicken  J. Env. Bio-Sci., (2015): Vol. 29 (2):509-51
  12. A.Pundlik, S.Doley, U.M.Tumlam, S.P.Awandkar, J.A.Kesharkar, P.A.Tembhurne, S.Gohain and V.C. Ingle. Isolation and Characterization of multi-antibiotic resistant E.coli from diarrhoeic companion dogs.Indian J. Field Vet(2015) 11: 14-15
  13. Suryawanshi S. N., A. Tembhurne, S. Gohain and V. C. Ingle . Prevalence of brucella antibodies in sheep and goats in Maharashtra Indian Res. J. Ext. Edu.(2014)14 (4): 75-77
  14. Kadam,A.S.,Tembhurne, A., Ingle,V.C. ,Manesh Kumar, P.,Dhok, A.K. and  Kalorey, D.R. Detection of sheep and goat pox viruses by polymerase chain reaction. Indian J. Field Vet. (2014) 9 (3) : 48-51
  15. Manesh Kumar ,P.,Raja,P., Dixit.M.M., Ingle,V.C.,Pawade, M.M.,Tembhurne, P.A. and Kalorey, D.R. Correlation of canine parvo virus infection with Escherichia coli in clinical cases. Indian J. Field Vet. (2014) 9 (3) : 46-47
  16. Pawade, M.M.,Ingle,V.C.,Tembhurne, P.A., Budhe,S.D.,Lahade,V.C., Manesh Kumar, P.and Kalorey, D.R Characterization, serotyping and antibiogram of E.coli isolated from fecal samples of Kites. Indian J. Field Vet. (2013) 9 (2) : 51-53
  17. Ingle,V.C., Tembhurne, P.A., Kamble, S., Undirwade, S., Ingle, R., Bhaisare, D., Wankhade, G.,Meshram, Y., Lahade,V.C., Pawade, M and  Kalorey, D.R Serotyping and antibiogram of E.coli isolates  recovered from Bats. Indian J.  Field Vet. 7(2012)7(3): 40-42
  18. A. Chopade, N. V. Kurkure, P. A. Tembhurne, A. G. Bhandarkar, S. S. Rautmare and D. R. Kalorey, Detection of Swine Fever Virus from Field Outbreaks in Maharashtra, Indian Vet. J.( 2011) 88 (10):62-63.
  19. Chopade N.A., Kurkure N.V. Tembhurne P.A. Handling of disease outbreak in poultry Poultry Line ( 2011) Feb 2011, Issue 2, 27- 32
  20. Tembhurne P,A., Mondal B, Pathak K.B., Biswas S.K., Sanyal A, Yadav M.P., Bandyopadhyay S.K. and Singh R. K. Segment-2 sequence analysis and cross-neutralization studies on some Indian bluetongue viruses suggest isolates are VP2-variants of serotype 23.Arch Virol. (2010). 155(1):89-95.
  21. Chopade N.A., Kurkure N.V. Tembhurne P.A., Bhandarkar A.G. and Kalorey D.R. Detection of 5’ non coding region (5’NCR) of Swine Fever Virus in tissue by RT-PCR. Indian J. Comp. Microbiol. Immunol. Infe. Dis. ( 2010).Vol. 31. No. 1&2 ( Jan –Dec), 69-70
  22. Ingle V.C, Suryavanshi S.N., Raut C.G., Chitamber S.D., Kalorey D.R. and Tembhurne A.     Rotaviruses of Animals. Animal Disease Bulletin  ( 2010)  Vol. 9 ( DIS Pune).  
  23. Hicks J ., Tembhurne A*., Liu H.C. Identification of microRNA in the developing chick immune organ. Immunogentics.  (2009) Mar; 61(3) :231-40. 
  24. Ingle,V.C.,Tembhurne, P.A., Pawde, M.M.,Jadhav, M.B.,Kamble, Lahade,V.C.andKalorey, D.R. Detection of canine parvo virus from fecal samples of dogs.Indian J. Comp. Immunol. Infect. Dis.(2009) 30 (2): 140
  25. Ingle,V.C.,Kamble,S.,Date,A.,Lahade,V.C.Tembhurne, P.A., Pawde, M.M.,Raut,C.G. and Kalorey, D.R. Characterization of Escherichia coli isolates from fecal samples of laboratory animals Indian J. Comp.  Immunol. Infect. Dis.(2009)30 (2): 139.
  26. B. Pathak, S.K. Biswas, P.A. Tembhurne, M. Hosamani, V. Bhanuprakash, Gaya Prasad, R.K. Singh T.J. Rasool, B. Mondal. Prokaryotic expression of truncated VP7 of bluetongue virus (BTV) and reactivity of the purified recombinant protein with all BTV type-specific sera. Journal of Virological Methods 152 (2008) 6–12
  27. Hicks J., Tembhurne P., Liu H. C. MicroRNAs expression in chicken embryos. Poultry Science. (2008) Nov; 87 (11):2335-43
  28. Mondal, A.K.Bera., M. Hosamani., Tembhurne P.A., S.K.Bandyopadhyay. Detection of Orf virus from an outbreak in goats and its genetic relation with other parapoxviruses .Vet Res Commun.  ( 2006).Jul;30(5):531-9
  29. Hosamani M., Mondal B., Tembhurne P.A., Bandyopadhyay S.K, Singh R.K, Rasool T.J. (2004) Differentiation of sheep pox and goat pox virus by sequence analysis and PCR-RFLP of P32 gene. Virus Genes  (2004) Aug.29 (1):73-80

Completed Research Projects:

Sr. No

Title of Project

Sponsoring Agency and duration

Total Outlay

(Rs in Lakhs)

1

 Cooperating centre Principle Investigator:  Understanding the molecular basis of peste-des-petits ruminants virus (PPRV) mediated host immune modulation of the development of next generation vaccines

ICAR-NASF ( 2017-2020)

 224 .00

49.12 (NVC centre )

2

Co-coordinator & PI & CCPI

 Molecular Surveillance and cross species transmission of Rotaviruses of animals and human origin from Maharashtra

ICMR, New Delhi (2017-2020)

50.00

3

Co-coordinator

DBT STAR college , NVC Nagpur

DBT,GOI New Delhi

2019-2021

82

4

Co-Investigator:

Clinical Evaluation of indigenous medication against mastitis & tick infestation among farm animals

National Innovation fund, Ahmadabad

2019-23

8.47

5

Principle Investigator:  Deciphering role of miRNA in chicken embryonic developmental pathology and its evaluation as therapeutics in immune suppressed chicks.

DST, GOI, New Delhi (2012-15)

18.00

6

Co-Investigator:

Molecular epidemiology of Brucellosis

DBT,GOI New Delhi

(2012-15)

114.7

7

Co-Investigator:

Epidemiological studies on classical swine fever in central India & Goa.

DBT, GOI, New Delhi

(2009-2012)

105

8

Co-Investigator:

“Centre for Zoonoses” under Niche Area of Excellence

ICAR-ICMR, New Delhi

(2014-2019)

435.00

 

List of On-going projects : Nil

List of On-going Agency projects : Nil

Agency project private funded partnership  :  

            Co-PI- Clinical evaluation of indigenous herbal medicine  with NIF, Ahmedabad ,Gujrat

List of MOU Completed – Nil

List of On-going MOU’s – mention duration / date of starting and end date

–  Nil

 List of PG Thesis completed

 

Sr. No.

Thesis title/ Dissertation title

Name of student

Name of guide

Year of completion
 

M.V.Sc

      

1

 In-ovo inhibition of gga-miR-142-3p and evaluation  of effect on targeted genes involved in signal transduction and immune response

Manesh Kumar  p.

Dr. P.A Tembhurne

2013

2

Role of miR-142-3p  in cell structural integrity and cell  migration in immune organs in chicken  embryo

Raja  P.

Dr. P.A Tembhurne

2013

3

Role of CD200 and TFFb2  genes in immunocsuppressed chicks in response to miRNA 142-3p therapy

Shabanam Kumari

Dr. P.A Tembhurne

2014

4

Development of Dried SAmple-Spot-Polymerase Chain Reaction (DSS-PCR) for detection of Peste Des Petitis Ruminants Virus

Mohan Anatrao  Pundlik

Dr. P.A Tembhurne

2015

5

Molecular Epidemiology of Peste-des-petits ruminant  virus in and around Nagpur

Narayan R

Dr. P.A Tembhurne

2016

6

Genetic analysis of Panthera Pardus  and Panther tigris by mtDNA PCR

Pooja Achrya

Dr. P.A Tembhurne

2018

7

Nucleocapsid gene based molecular characterization of Peste Des Petitis Ruminants Virus

Priyanka B. Pharande

Dr. P.A Tembhurne

2018

8

Studies on miRNA expression in Peste-des-petits ruminant  virus  infected goats

Preeti  P. Bramhapurkar

Dr. P.A Tembhurne

2018

9

Studies on miRNA Expression in Peste-des-petits ruminant  virus   and genes  associated with immune response and immunity

Pragti Bhoyer

Dr. P.A Tembhurne

2019

10

In vitro studies on miRNA and genes in response to small Ruminant   morbillivirus virus infections

Anuradha A. Nemade

Dr. P.A Tembhurne

2021

11

Nonstructural gene based molecular characterization of Bluetongue virus

Deepali Gharat

Dr. P.A Tembhurne

2022

12

Development of   Anti-BTV antibody detection platform using recombinant VP7 protein

Aniket Thakare

Dr. P.A .Tembhurne

2023
 

PhD

     

1

Development of trusted Infectious Bursal Disease Virus VP2 protein based diagnostics antigen and its efficacy as candidate immunogens

Sudhakar Awandkar

Dr. P.A.Tembhurne

2019

2

Studies on Molecular profiling of Bovine Rota viruses

Anand S. Kadam

Dr. P.A.Tembhurne

2020

Brief report of Completed research project:

Project : Deciphering role of miRNA in chicken embryonic developmental pathology and its evaluation as therapeutics in immune suppressed chicks.

Brief report :

1. The  gga-miR-142-3p plays a vital role in the embryonic developmental stages of the chicken     regulating the genes involved in signal transduction, immune response in the immune organs and other organs.

2)Aberrant expression of the gga-miR-142-3p during the embryonic stages disturbs the environment of the immune organs and may lead to immune compromised chickens

 3)The gga-miR-142-3p miRNA therapy was contributing in the restoration of the hematopoiesis in the immune suppressed chicks and   immunosuppression led to pathology was recovered in response to the miRNA therapy.

4)   The study also delineated that the effect of miR-142-3p on the migration on Bu-1 specific B lymphocytes and their restoration clearly indicating the remedial outcomes in  all the organs.

5) The study  indicated the role of gga-miR-142-3p on direct regulation of the target genes and their contributing effect on cell structural integrity, cellular development, cell cycles, cell migration, immune responsive genes regulation etc.
     

Project : Understanding the molecular basis of peste-des-petits ruminants virus (PPRV) mediated host immune modulation of the development of next generation vaccines

 Brief Report:

1.The transcriptomic data revealed 682 differentially expressed genes out of 12688 genes analysed by transcriptomic analysis of PBMC from PPRV infected goats.

2. The the  genes associated with immune response and immunity  viz. IL18RAP , ISG20 , CD93 , CD96 and ITK  on analysis in isolated PBMC showed the  genes were differentially expressed in infected, vaccinated and apparently healthy animals , correlated with transcriptomic data.

3. The miRNAs profiling in infected , vaccinated  and apparently healthy goats  revealed the differential expression, which has the potential for differentiation of vaccinated vs infected animals.

4. The miRNAs profile in serum and in PBMC are varied indicating contribution from different tissue. 

5. The circulatory miRNAs  miR-21 , miR-211, miR-34 and  miR-193, miR-486 was found to differentially expression, and miR-21 was correlating the disease progression.

6. In vitro PPRV infections studies indicated the differential expression of immune related genes in time dependent manners correlating with natural infections.
     

Project: Molecular Surveillance and cross species transmission of Rotaviruses of animals and human origin from Maharashtra

Brief Report:

1.     The rapid test like latex agglutination test (LAT), Lateral flow and RNA-PAGE Useful for    preliminary screening of rotavirus.

2.     VP6, VP4 and VP7 gene based RT-PCR detection was useful in confirming occurrence    rotavirus.

3.     Genotypes analysis for each individual samples rotavirus indicated variation in both G and P     types of animal rotavirus and human rotavirus.

4.     Among G genotypes, G10 in cattle & buffalo calves, G2 and G10 genotype in piglets and inhuman infants G8 was recorded. Among P genotypes, P[5] was the observed in cattle calve, P[11] in piglets and human infants was recorded. G10P [5] combination was found in cattle calves.

5.     Based upon sequencing and Phylogenetic analysis of VP6,VP4 and VP7 Positive samples showed interspecies cross transmission relationships. Sequencing analysis revealed varying degree of similarity with world and Indian isolates.

Research Recommendations

NVC/Nagpur/Microbiology/02/2015-16 ( XIII and XiV JASRC  Research Recommendation 2014-15 and 2015-16)

Investigator :Dr.  Mohan Pundlik  and  P.A. Tembhurne

Ink-free news paper or filter paper of around 1.5cm diameter spotted with nasal secretion of affected animal was found suitable for diagnosis of Peste des Petits Ruminants by RT-PCR

( XV JASRC  Research Recommendation16-17) 

Investigator : Narayan R.and Tembhurne P.A.

 The present study recommended that for detection of PPRV in field samples  by RT-PCR techniques targeting both the M and N genes should be employed.

( XVII JASRC  Research Recommendation18-19)

 Investigator Dr. Pooja Acharya,Dr. P.A.Tembhurne, Dr. V.M.Dhoot and Dr. G.R.Bhojne

It is recommended that PCR techniques is useful for amplification of cytochrome-b gene of tiger and leopard  and its sequencing from the scat samples of feces for its differential identification of feline species.

( XVIII JASRC  Research Recommendation19-20)

 Investigator  Sudhakar Awandkar ,P.A.Tembhurne, V.C.Ingle, N.V.Kurkure,S.W.Bonde and S.P.Chudhari

It is recommended that to use silver nanoparticle adjuvanted  Infectious bursal disease virus  recombinant VP2 protein  for inducing  significant  immune response in chicken

The Infectious bursal disease virus  recombinant marker VP2 immunogen  is suitable for differentiating  infected verses  vaccinated chicken.

The Infectious bursal disease virus  recombinant  marker VP2 protein based dot blot   assay is a suitable rapid DIVA test  for differentiating  infected chicken from those vaccinated with marker vaccine.

( XVIII JASRC  Research Recommendation19-20)

Investigator : P.A. Tembhurne

It is recommended that  screening of the battery of miR-34, miR-193,miR-346, miR-486 and miR-211  and  ILR18 and ISG20 genes were suitable for differentiation of PPR Infected vs Vaccinated animals.

( XIX JASRC  Research Recommendation21-22)

Investigator : Dr.A.S.Kadam, Dr.P.A. Tembhurne  Dr. S.P. Chudhari  Dr. N.V.Kurkure, and  Dr. V.C.Ingle,

It is recommended that the  sequence based genotyping should be performed to confirm the rotavirus genotypes which are untenable by PCR.

 

 

 

 

Extension-:

1 College Publications

2 Leaflets  ::    on   Lumpy skin Disease , Naishargeek Aushadhi  vanspaticha upyog karun guranmadheel Gochid Nirmulan ,

3 Folders :  on    Mastits ,  Control of tick Infestation through polyherbal indigenous medication and on “Naishargeek Aushadhi  vanspaticha upyog karun guranmadheel Gochid Nirmulan,

4 List of Adopted villages –photographs etc..

             Worked in adopted village  from  2009- 2013 at  Bajargaon  and  from 2013- 2017  Borgaon Dist. Nagpur ,, Working since as per need to Deliver lectures, Demonstrations for farmers and Livestock health workers etc. 

  1. Success story (Audio/Video) : Nil
  1. 6. Year wise Extension activities :
   

2009 to 2013 :

worked as Bajaragon  and different  camps arranged under NSS and other    health camps carried out at Bajargaon as well as other locations where extension activities      likes awareness on mastitis , vaccination , clean milk production, viral disease management     etc was carried out .

20014 to 2017:

worked at adopted village Boregaon Dist. Nagpur and other adjacentvillage  as well as NSS camps and other health camps where clean milk production, Animal health and vaccination, mastitis and its management, CMT test at farmers door steps etc    awareness and other allied activities where done and services for disease diagnosis and Antibiotic sensitivity test was carried out.

2018 to 2024 :

Health Camps , NSS camps  and other camps  were attended , delivered lectures Demonstration on CMT, clean milk production , vaccination , services provided for disease diagnosis.  Work as LSD expert during LSD epidemic and also  as LSD lab In-charge  for LSD diagnosis-

LSD Warrior

Work as core team  member COVID diagnosis -COVID warrior during Covid-19 pandemic
  1. 7. Extension Literature:

 in the form of article in various local,  hindi and English magazines as published or online content on Peste des petits ruminants disease, Lumpy skin disease, miRNA  etc

  •  Achievements of the Department:

    • Infrastructure development for Education and Research in Veterinary Microbiology & Animal Biotechnology (UG,PG & PhD)
    • Development of HRD in Animal Biotechnology through national programme.
    • Establishment of Laboratory Animal Experimental Facility.
    • Repository of bacterial & viral isolates viz. Staphylococcus aureus, Listeria monocytogenes, E. coli and Parvovirus, Poxviruses, IBD etc
    • Development of in-house assays for diagnosis of infectious diseases viz. Sample collections (Dried spot sample PCR using newspaper /filterpapers) –DSS PCR); Multigene based identification of PPRV for better confirmation of disease in Indian scenario).
    • Antigen detection of Parvovirus by Multiplex PCR
    • Molecular epidemiology of Staphylococcus spp., Listeria, spp. Rotaviruses and Poxviruses, PPR, and IBD from Maharashtra State.
    • Identified herbs for neutralization and inhibition of aflatoxin and ocrotoxin A.
    • Deciphered role of miRNA in therapy of immunocompromized chicken.
    • Development of Milk (Dot, Indirect & AB) and Serum(Indirect) ELISA for detection of L. monocytogenes
    • Isolation & characterizations of Listeria from wild life
    • Identify Disinfectant  to reduced contamination of L. monocytogenes at bovine farm.
    • Several Gene Sequence  submitted  to gene bank

     Facility created:

    • High end research facilities viz. equipments ( Real time PCR, Thermal Cyclers, Hybridization oven, Electrophoresis systems,  Biosafety cabinets, CO2 incubators,   etc)
    • Establishment of Laboratory Animal Experimental Facility.
    • Cell culture facility for virology works
    • Molecular biology facility ( DNA/RNA/Protein works)

     

    Scientific Collaborations 

    • Central India Institute for Medical Sciences, Nagpur (MOU signed)
    • Apex research institutes in Veterinary Sciences, like IVRI, Izatanagar, NIVEDI, Bangalore, National Institute of Virology, Pune etc
    • Indira Gandhi Government Medical College, Nagpur
    • Government Medical College, Nagpur
    • Mahatma Gandhi Institute of Medical Sciences, Sewagarm, Wardha
    • Veterinary Colleges of different states
    • IISER, Mohali
    • Delhi University (South Campus)
    • Indian Immunologicals

  1. Vision/Mission  of the Department:

    To impart cutting edge U.G. and P.G. and Ph.D. teaching to create efficient Human Resource in Veterinary Microbiology and Animal Biotechnology

    To establish Centre for Animal Disease Diagnosis and Research

    To establish technical linkage with national and international scientific organizations for better academics and research.

    To explore research in functional genomics especially in small RNA biology and its application for future therapeutics

    To cater services to state government officers, livestock owners through extension activities.

  1. Course Offered / Conducted by the Department:

     U.G.  course :  Veterinary Microbiology ( MSVE -2016)  3+2=5 II nd Year of BVSc.& AH

     P.G.Courses: (In Vety.Microbiology)

    Sr. No.

    Course  No.

    Title

    Credits

    Semester

    1

    VMC-601

    Bacteriology -I

    3+1=4

    I

    2

    VMC-602

    Bacteriology -II

    3+1=4

    II

    3

    VMC-603

    Veterinary Mycology

    1+1-2

    II

    4

    VMC-604

    General Virology

    2+1=3

    I

    5

    VMC-605

    Systematic Animal Virology

    3+1=4

    II

    6

    VMC-606

    Principles of Immunology

    2+1=3

    I

    7

    VMC-607

    Vaccinology

    2+0=2

    III

    8

    VMC-608

    Diagnostics of Infectious Diseases

    1+2=3

    III

    9

    VMC-609

    Techniques in Microbiology & Immunology

    0=3=3

    I

    10

    VMC-691

    Masters Seminar

    1+0=1

    III

    11

    VMC-699

    Masters Research

    20

    III & IV


    P.G. Courses : (In Animal Biotechnology)

    Sr. No.

    Course  No.

    Title

    Credits

    Semester

    1

    ABT-601

    Basic & Applied  Biotechnology

    3+0=3

    I

    2

    ABT-602**

     Fundamentals of Cell & Molecular Biology

    3+0=3

    I

    3

    ABT-603

    Applied Molecular Biology

    2+1-3

    II

    4

    ABT-604**

    Animal Cell Culture; Principles & Applications

    1+2=3

    I

    5

    ABT-605**

    Molecular Diagnostics

    1+2=3

    II

    6

    ABT-606

    Vaccine Biotechnology

    2+0=2

    III

    7

    ABT-607

    Immunology Applied to Biotechnology

    1+1=2

    III

    8

    ABT-608

    Introduction to Bioinformatics

    1+1=2

    III

    9

    ABT-609**

    Animal Genomics

    2+1=3

    II

    10

    ABT-610**

    Reproductive Biotechnology

    2+1=3

    III

    11

    ABT-611**

    Techniques in Molecular Biology and Genetic Engineering

    0+3=3

    II

    12

    ABT-613

    Molecular Forensics

    2+1=3

    III

    13

    ABT-616

    Animal Biotechnology

    0+3=3

    II

    14

    ABT-691

    Masters Seminar

    1+0=1

    III

    15

    ABT-699

    Masters Research

    20

    III & IV

    ** Compulsory courses

    Ph.D.Courses: (In Vety.Microbiology)

    Sr. No.

    Course  No.

    Title

    Credits

    Semester

    1

    VMC-701

    Advances in Bacteriology

    2+1=3

    I

    2

    VMC-702

    Advances in Mycology

    2+1=3

    I

    3

    VMC-703

    Bacterial Genetics

    2+1=3

    I

    4

    VMC-704

    Microbial Toxins

    2+1=3

    II

    5

    VMC-705

    Molecular Determinants of Bacterial Pathogenesis

    2+1=3

    II

    6

    VMC-706

    Advances in Virology

    2+1=3

    I

    7

    VMC-707

    Molecular and Genetic Aspects of Viral Pathogenesis

    2+1=3

    II

    8

    VMC-708

    Structure Function Relationship of DNA and RNA Viruses

    3+0=3

    II

    9

    VMC-709

    Oncogenic Viruses

    2+0=2

    III

    10

    VMC-710

    Slow Viral Infections and Prions

    2+0=2

    III

    11

    VMC-711

    Molecular Immunology

    2+1=3

    I

    12

    VMC-712

    Advances in Cellular Immunology

    2+1=3

    I

    13

    VMC-713

    Cytokines and Immunomodulators

    2+0=2

    II

    14

    VMC-714

    Advances in Vaccinology

    2+0=2

    III

    15

    VMC-715

    Advances in Immunodiagnostics

    1+1=2

    III

    16

    VMC-716

    Modern Immunotechnology

    1+2=3

    III

    17

    VMC-717

    Current Topics in Infection and Immunity

    3+0=3

    III

    18

    VMC-718

    Veterinary Microbial Biotechnology

    2+1=3

    II

    19

    VMC-790

    Special Problem

    0+2=2

    IV

    20

    VMC-791

    Doctoral Seminar I

    1+0=1

    II

    21

    VMC-792

    Doctoral Seminar II

    1+0=1

    III

    22

    VMC-799

    Doctoral Research

    45

    		 

Departmental Research Activity :

Research programmes:

i) Completed Research Projects

Sr. No.

Type

Principle

Investigator

Title

Period

Cost

(Rs in Lakhs )

1.

NATP(MM)

Dr. D.R.Kalorey

Dr.V.C.Ingle

Animal Health information system through disease monitoring and surveillance

2000-04

19.80

 

2.

NATP(CGP)

Dr. D.R.Kalorey

Dr.V.C.Ingle

Development of ELISA for detection of L. monocytogenes from milk.

 

2002-05

14.09

3.

DBT Network

Dr. D.R.Kalorey

Development of Standardized herbal product for bovine mastitis

2006 -11

43.22

4.

DST(SERB- Young Scientist)

Dr.S.R. Warke

Genetic diversity of Listeria monocytogenes with different virulence potential  

2010-13

10.00

5.

Indo German ICMR

Dr.D.R.Kalorey

Dr.N.V.Kurkure

Dr.S.R. Warke

Virulence profiling of Listeria monocytogenes isolated from food, veterinary and clinical sources.

2011-13

10.66

6.

DBT

Dr.D.R.Kalorey

Dr. N.V. Kurkure

Dr. P.A. Tembhurne

Dr.ShubhangiWarke

Dr. S.P.Awandkar

DBT Network Project on Brucellosis.

 

2012-16

114.70

7.

DST

Dr. P.A. Tembhurne

Deciphering role of miRNA in chicken developmental pathology & its evaluation as therapeutic in immune suppressed chicks.

2012-15

18.00

8.

DBT-HRD

Dr.V.C.Ingle

Development HRD in Animal Biotechnology.

2010-onwards

109.02

9.

DBT

Dr.D.R.Kalorey

Dr.N.V.Kurkure

Dr. S. R. Warke

Translational research on Molecular epidemiology of L. monocytogenes.

 

2012-2017

57.70

10.

ICAR-TSP

P. A. Tembhurne

( Co-PI)

Establishment of community based animal husbandry practice in tribal villages of Gadchiroli district of Maharashtra State

 

2014-2017

206.00

11

ICAR-ICMR

Dr.V.C.Ingle

( Co-PI)

P. A. Tembhurne

( Co-PI)

Dr.S.P.Awandkar

( Co-PI)

Centre for Zoonoses

(Niche Area of Excellence)

2015-2019

407.00

12

ICAR NASF

Dr. P.A.Tembhurne  (Co-operating centre PI)

Understanding the molecular basis of peste-des-petits ruminants virus (PPRV) mediated host immune modulation for the development of next generation vaccines

2016-2019

300.00

13

ICMR

 Dr. V.C. Ingle

Molecular  epidemiology and cross species transmission of rotavirus of animal and Human origin from Maharashtra

2017-2020

70.00

  

ii)Ongoing Research Projects:

Sr.

No.

Type

Principle Investigator

Title

Period

Cost (Rs in lakhs)

1

DBT

Department of Veterinary Microbiology

Star College Scheme of DBT

2020-2022

19.00

 

ii) Research Projects submitted to various funding agencies

Sr. No.

Title of Project

Investigators

Duration/

Date of submission

Funding Agency

Total Outlay

(in lakhs)

1.

Molecular epidemiology & development of multivalent vaccines against Bovine Mastitis caused by Staphylococcus aureus

Dr.D.R.Kalorey

3 yrs

MBC

221.70

2.

Development of Multiple Dot –ELISA for simultaneous diagnosis of reproductive diseases.

Dr.D.R.Kalorey

3 yrs

MBC

102.21

3.

Molecular characterization of Avian pathogenic Escherichia coli  

Dr.D.R.Kalorey

3 yrs

MBC

192.10

4.

Molecular characterization of Brucella strains from reproductive disorders of  cattle, buffalo and goat

Dr. V.C.Ingle

3 yrs

MBC

90.56

5.

Development of standardized vaccine for Staphylococcal bovine mastitis in India.

Dr.D.R.Kalorey

3 yrs

BIPP

12.119744 Crores (2.747624 crores)

6.

Development of vaccine against bovine mastitis caused by CNS

Dr.D.R.Kalorey

3 yrs

RGS& T C

67.14

7.

Development of multiplex PCR for detection of enterotoxigenic Staphylococcus aureus from milk and milk products  

Dr.D.R.Kalorey

3 yrs

RGS& T C

59.14

 

8.

Molecular Epidemiology and development of vaccine against bovine mastitis

Dr.D.R.Kalorey

3 yrs

DBT Network

612.24

9.

Central Disease Diagnostic Laboratory

Dr.D.R.Kalorey

3 yrs

 

ICAR

56.00

 

10.

Deciphering role of miRNA in chicken embryonic developmental pathology and its evaluation as therapeutics in immune suppressed chicks.  

Dr. P.A.Tembhurne

3 yrs

DST-FAST TRACK

24.672

11.

Evaluation of antiviral activity of Growell liquid against Fowl pox virus.

Dr. P.A.Tembhurne

6 months

 

Growell India

1.20

12.

Centre for Animal Disease Diagnosis, Research and Control

Dr.D.R.Kalorey

5 Years

RKVY

461.50

13.

Safety and immunogenicity of Brucella abortus RB51 vaccine in  cattle and buffaloes

 

Dr.D.R.Kalorey

3 Years

DBT

55. 648

14.

Development of serological and DNA based diagnostic tools for brucellosis

Dr.D.R.Kalorey

DBT

DBT

71.686

15.

*miRNA profiling  of canine  transmissible venereal tumor : A novel markers  in body fluids for early diagnosis of cancer

Dr. P. A. Tembhurne

Dr.V.C.Ingle

Dr. S. P. Awandkar

Dr.G.R.Bhojne

 

3Yrs

DBT

32.00

16

Molecular markers to identify pathogenic Listeria monocytogenes from animal food origin & human clinical cases.

Dr. S.R. Warke

Dr.V.C. Ingle

Dr. N.V. Kurkure

3 Yrs

11-4-2014

DBT

48.84

17

Development of Infrastructure Facility for Animal Disease Diagnosis and Molecular Characterization of Pathogens

Dr.V.C.Ingle

Dr. P. A. Tembhurne

Dr. S. P. Awandkar

Dr.U.M.Tumlam

Dr.S.R.Warke

Dr.V.K.Basunathe

Dr.G.R.Bhojne

Dr.P.M.Sonkusale

3Yrs

13-10-2015

RKVY

282.8

18.

*Decipering molecular interaction of Infectious Bursal Disease Virus (IBDV) with cellular genes and host encoded miRNA

Dr. P. A. Tembhurne

 

21-10-2015

ICAR

 

19.

Studies on Molecular Epidemiology of Pestiviruses in small ruminant

Dr.V.C.Ingle

2 yrs

11-9-2015

 

 

 

 

ICAR Ad-hoc

40 .00

20.

Epidemiology and Impact Assessment Of Infectious Bovine Rhinotrachitis in Livestock of Central India

Dr.S.P.Awandkar

 

2 yrs

11-9-2015

 

 

 

ICAR Ad-hoc

40 .00

21.

Studies on cross species transmission of Rotaviruses in livestock

Dr.V.C.Ingle

6 m

8-2-2016

NAAS short term study

1.00

22

Disinfectant Resistant of L.monocytogens from bovine environment

Dr.S.R.Warke

6 m

8-2-2016

NAAS short term study

1.00

23.

Status of clean milk production at organized and non-organized farms in Bhandara district

Dr.S.P.Awandkar

6 m

8-2-2016

NAAS short term study

1.00

24.

Curing of plasmid from CPS & CNS isolated from Bovine Subclinical Mastitis using Kamdhenu ark

Dr.U.M.Tumlam

6 m

8-2-2016

NAAS short term study

1.00

25.

Evaluation of cheapest cellular matrix base materials viz. News paper/filter paper for room temperature PPR sample dispatch and its molecular characterization

Dr.P.A.Tembhurne

6 m

8-2-2016

NAAS short term study

1.00

26.

Holistic approach to identify common infectious agents of diarrhea in dogs

Dr.P.A.Tembhurne

21-6-2016

DBT

 

27.

Studies on Molecular epidemiology of IBR virus in cross bred bovines of Maharashtra state

Dr.V.C.Ingle

3 Years

11-7-2016

RGSTC

20.00

 

 Agency Projects undertaken by the Department 

Sr. No.

Agency

Principle

Investigator

Title

Period

Cost (Rs)

1.

Growell India Pvt.Ltd. Pune

Dr.D.R.Kalorey

Role of Growell as immuno modulator in IBD vaccinated Chicks.

1997

18,950/-

 

2.

Growell India Pvt.Ltd. Pune

 

Dr.D.R.Kalorey

Immunomodulatory effect of Growell during experimental Aflatoxicosis in chicks.

1997

26,880/-

 

3.

DaburAyurvet Ltd. Gaziabad -10

Dr.D.R.Kalorey

Effect of herbomineral premix on fungal Growth & in vitro inhibition of aflatoxin B1 production.

1998

30,500-/

 

4.

DaburAyurvet Ltd. Gaziabad –10

Dr.D.R.Kalorey

Effect of herbomineral toxin binder during experimental mycotoxicosis in broiler chicks.

2000

30,500/-

 

5.

Kemin Nutriti onal Technolo gies,(India)

Dr.D.R.Kalorey

Efficacy of Toxin-binder against aflatoxicosis in  broilers.

2002

30,000/-

6.

Dabur Ayurvet Ltd

Gaziabad – 10

Dr.D.R.Kalorey

In vivo efficacy of herbal preparation during simultaneous mycotoxicosis in Broilers.

2002

 15,000/-

 

7.

Dabur Ayurvet Ltd

Gaziabad – 10

Dr.D.R.Kalorey

.Evaluation of efficacy of mastilep during subclinical mastitis

2002

10,000/-

8.

M/s Intervet Pvt. Ltd.,Pune    

Dr.D.R.Kalorey

Evaluation of Brucella RB 51               vaccine under field condition.   

2005    

60,000/-

9.

M/s Intervet Pvt. Ltd.,Pune    

Dr.D.R.Kalorey

Evaluation of safety and efficacy of oil and gel adjuvanted BOVILS (R) HSBQ vaccine in cattle under field condition.

2007

25,000/-

10.

Bentoli Agri Nutrition Asia Pvt. Ltd

Dr.D.R.Kalorey

Evaluation of antibacterial and antifungal activity of antimicrobial product.

2007

1,65,865/-

11.

Bentoli Agri Nutrition Asia Pvt. Ltd

Dr.D.R.Kalorey

Antifungal and antimycotoxic activity of mold inhibitor

2007

38,080/-

12.

Indian Immunologicals Limited

Dr.D.R.Kalorey

To study Safety and immunogenicity of Brucella abortus RB51  in  cattle

2009

48,750/-

13.

Cattle Remedies, India Ltd.

Dr.D.R.Kalorey

Evaluation of Mastodin in bovine mastitis

  2011

80,000/-

14

IIL,

Hyderabad

Dr.S.R.Warke

An open label multicentric clinical trial to study safety and immunogenicity of Tetanus toxoid vaccine in animals

2019-2020

2.29

 

 

Recommendations:

Sr. No.

Project No.

 

Research Workers

Recommendations

 1

NVC/Micro/02/2004

Dr. D.R.Kalorey

Dr.Jyoti Bodade

Chicks vaccinated at 7th day of age with inactivated emulsified ND+IBD vaccine containing  10%  &  20% Vit E are protected  against New Castle Disease.

 

 2

NVC/Micro/01/2007

Dr. D.R.Kalorey

Dr.P.K.Jallewar

Norfloxacillin & Chloramphenicol can be used in Listeria infection, since the prevalence of Listeria species  in fresh water fish Vagur (Clavrus batracus) was 19.50% and of Listeria monocytogenous  13.00 per cent.

 

 3

NVC/Micro/02/2007

Dr. D.R.Kalorey

Dr. Deepti  K.Pillai

Antigen capture Sandwich ELISA developed for the detection of Pasteurella multocida antigen is sensitive (81.25%) specific ( 100%) and easy to perform, hence can be used as an efficient diagnostic tool for the diagnosis of fowl cholera from serum.

 

 4

NVC/Micro/02/2015-16

Dr. P.A Tembhurne

Dr. M.A.Pundlik

Ink free news paper or filter paper of around 1.5cm diameter spotted with nasal secretion of affected animals was found suitable for diagnosis of Peste des Petits Ruminants Virus   by RT-PCR.

 

 5

NVC/Micro/02/2016-17

Dr. P.A Tembhurne

Dr. R. Narayan

For Detection of PPRV (Peste des Petits Ruminants Virus) in field samples, RT-PCR  technique targeting both the M & N gene should be employed.

 6

NVC/Micro/     /2018-19

 

 

Dr.Shubhangi Warke                       

The disinfectant Didocyldiamethyl ammonium chloride (DAC), found to be most effective against the L. monocytogenes, lower that the recommended concentration

 

 

 7

NVC/Micro/     /2018-19

 

 

 

Dr. P.A Tembhurne

Dr. Pooja Acharya  

The  multiplex PCR approach  for amplification of cytb of tiger & leopards   and its sequencing  from scat sample easily obtained  sample in wild as well as captivity will be a tool  useful for identification  of endangered species

 

Publications:

1) Scientific research paper published International    : 11, National   : 127

2)Research paper presented at seminar International  : 15, National :117        

3)Popular article : 42

4) Prepared Manual  on  Milk Dot ELISA for detection of    L. monocytogenes.

5)  Prepared Manual  on  Milk  Indirect  ELISA for detection of    L. monocytogenes.

6) Prepared Manual  on  Milk Avidin Biotin  ELISA for detection of    L. monocytogenes.

7) Prepared Manual  on  Serum ELISA for detection of    L. monocytogenes. 

8) Prepared SOPs of  Diagnostic protocol

9) Prepared Monograph: 05

10) Prepared Leaflets:10

 

B) Area of Research

Seminar/Conference / Organized:

Sr. No.

Seminar/Conference /Training organized

Date

1.

IX Annual Conference of Indian Association for the Advancement of  Veterinary Research (IAAVR) and National Symposium on “Concepts in Sustainable Livestock Production  and Health in New Millennium” 

4-5th Feb 2002

 

2.

 

National symposium on “Zoonoses and Biotechnological Applications”

 

4-5th  Feb.2008

 

3.

30th Annual Convention of Indian Association of Veterinary Microbiologists Immunologists and Specialist in Infectious Diseases (IAVMI) and National Symposium on “Challenges in Animal Health for Higher Productivity and Income to Farmers”

 

10-12th Feb 2017

 

 

Training/ Workshop/Popular Lecture

 

Sr. No.

Seminar/Conference /Training organized

Date

1.

Popular Lecture Series on Biotechnology.

 

4th  Oct.2007

2.

Work shop on “Dairy Management & Nutrition”

 

24th Aug. 2007

3.

Short term training course on “Application of Molecular Methods in Livestock Disease Diagnosis and Research”

 

17th-31st  July,2008

 

4.

 Short term Training course on “Diagnosis of Brucellosis” under the Continuing Veterinary Education Programme funded by Veterinary Council of India.

 

25-28th May 2009

5.

Short term Training course on “Diagnosis of Brucellosis” under         the Continuing Veterinary Education Programme funded by Veterinary Council of India,  New Delhi.

 

1-4th June 2009

 

6.

 

Three day workshop on “Care, Breeding and Management of Laboratory Animals.

 

15-17th Jan 2009

 

7.

Work shop on Bioinformatics and PCR. 

19-20thMarch 2010

 

8.

One day workshop on ”Dugdhotpadan: Krishi Jivnacha Shashwat Aadhar.

 

26th Dec 2014

9.

Entrepreneurial Seminar on “Large scale production of anti snake venom”

 

6th June 2015

 

 

Diagnostic services catered 

Diagnostic services are catered on payment basis with the following charges.

 Sr.No.       

Diagnostic Tests

Amount (Rs)

  1.

Cultural Examination & AST

200.00

  2.

Biochemical characterization of bacteria

200.00

  3.

Smear examination

100.00

  4.

Skin/hair for mycotic infection

300.00

  5.

Mycotoxin in feed

500.00

  6.

Brucellosis by RBPT/Salmonellosis by PAT

50.00 / Sample

  7.

Brucellosis by STAT

100.00/ Sample

  8.

Brucellosis by ELISA

500.00/ Sample

  9.

Tuberculin/Johnin test

200.00 / animal

  10.

Seromonitoring (RD)

500.00 / 5 sera

  11.

MPN

200.00

  12.

Plate count

300.00

  13.

CMT reagent

250.00/ 500 ml

  14.

Subclinical mastitis

20.00

  15.

Attending diseases outbreak & investigation

2000.00

  16.

Consultancy during outbreak / Visit

500.00 

Through Lectures, visit at farmers doorsteps, camps, exhibitions, rallies, adopted village, consultancy, Marathi article published in local Newspapers for farmers, Preparing leaflets and Pampltes for farmers ,through toll free services at university level as well as at department etc