Biotechnology Department

Academic:

1. Degree Offered: M.V.Sc. & Ph.D.
A. Title of degree: M.V.Sc. Veterinary Biotechnology
Duration: 2 years
Eligibility Criteria: B.V.Sc & A.H. with ICAR entrance
Intake Capacity: 01
Opportunities: Industries, Diagnostics, Academics, Research
B. Title of degree: Ph.D. Veterinary Biotechnology
Duration: 3 years (fresh candidates) and 4 years (In-service candidates)
Eligibility Criteria: M.V.Sc (Animal/Veterinary Biotechnology)
ICAR/MAFSU entrance
Intake Capacity: 01
C. Opportunities: Industries, Diagnostics, Academics, Research
2. Academic Regulations: MAFSU Academic Regulations 2022 – Post Graduate (P.G. &
Ph.D.) Degree Program
3. Admissions:
List of Admitted Students – First Year to Final Year (Veterinary Year wise / Fishery and Dairy Semester wise):

Sr. No. Degree Name of Student Enrl. No. Email Address Name of Advisor
1 M.V.Sc Ms. Shruti S. Barbuddhe V/18/030 barbuddhess06@gmail.com Dr. S. A. Ingle
2 Ph.D. Dr. Sujit M. Kolangath V/23/438 sujitkolangath@mafsu.in Dr. S. A. Ingle

4. Course offered: UG, PG , PhD – Semester / Year wise
 List of UG Courses (B.V.Sc & AH ) As per latest MSVE Guidelines), B.Tech. (D.T.) and
B.F.Sc as per ICAR – V Deans Committee – 2016. Not Applicable

Sr No Course No. Title Credit Course offered in the Year

List of M.V.Sc. Courses:

Sr. No Course No. Title Credits Core/ Optional
SEMESTER I
1 BTY 601 Basic and Applied Biotechnology * 2+0 Core
2 BTY 602 Fundamentals of Cell Biology * 2+0 Core
3 BTY 604 Animal Cell Culture–Principles and Applications 2+1 Optional
4 BTY 606 Immunology Applied to Biotechnology 2+1 Optional
Total 4+0=4
SEMESTER II
1 BTY 603 Molecular Biology and Genetic Engineering 2+0 Optional
2 BTY 605 Molecular Diagnostics 2+1 Optional
3 BTY 607 Introduction to Bioinformatics 2+1 Optional
4 BTY 608 Animal Genomics * 2+1 Core
5 BTY 610 Reproductive Biotechnology * 2+1 Core
Total 4+2=6
SEMESTER III
1 BTY 609 Techniques in Molecular Biology and Genetic Engineering * 0+2 Core
2 BTY 611 Master’s Seminar-I 1+0 Core
3 BTY 612 Master’s Research 0+10 Core
4 PGS 601 Technical Writing and Communication Skills 0+1 Core
5 PGS 602 Agricultural Research, Research Ethics and Rural Development Programmes 1+0 Core
6 PGS 603 Basic Concepts in Laboratory Techniques 0+1 Core
7 PGS 604 Intellectual Property and its Management in Agriculture 1+0 Core
8 PGS 605 Library and Information Services 0+1 Core
Total 18
SEMESTER IV
1 BTY 612 Master’s Research 0+20 Core
Total 20

List of Ph.D. Courses:
In-Service

Sr. No Course No. Title Credits Core/ Optional
SEMESTER I
1 BTY 701 Genetic Engineering* 1+2 Core
2 BTY 709 Advances in Animal Cell Culture* 2+1 Core
Total 3+3=6
SEMESTER II
3 RPE 700 Research and Publication Ethics 1+1 Core
4 BTY 702 Functional Genomics and Proteomics 3+0 Optional
5 BTY 703 Advances in Cell and Molecular Biology 2+0 Optional
6 BTY 704 Diagnostic Platform 1+1 Optional
7 BTY 706 Trends in Vaccinology 2+1 Optional
8 BTY 707 Advances in Bioinformatics 1+1 Optional
Total 1+0=1
SEMESTER III
9 BTY 712 Doctorate Seminar-I 1+0 Core
10 BTY 713 Doctorate Seminar-II 1+0 Core
11 BTY 705 Gene Manipulation and Genome Editing 2+0 Optional
12 BTY 708 Advances in Reproductive Biotechnology 2+1 Optional
13 BTY 710 Industrial Biotechnology 2+1 Optional
14 BTY 711 Rumen and Feed Biotechnology 2+1 Optional
Total 2+0=2
SEMESTER IV
15 BTY 714 Doctorate Research 0+15 Core
Total 15
SEMESTER V
16 BTY 714 Doctorate Research 0+15 Core
Total 15
SEMESTER VI
17 BTY 714 Doctorate Research 0+15 Core
Total 15
SEMESTER VII
18 BTY 714 Doctorate Research 0+15 Core
Total 15
SEMESTER VIII
19 BTY 714 Doctorate Research 0+15 Core
Total 15

Regular

Sr. No Course No. Title Credits Core/ Optional
SEMESTER I
1 BTY 701 Genetic Engineering* 1+2 Core
2 BTY 702 Functional Genomics and Proteomics 3+0 Optional
3 BTY 703 Advances in Cell and Molecular Biology 2+0 Optional
4 BTY 704 Diagnostic Platform 1+1 Optional
5 BTY 711 Rumen and Feed Biotechnology 2+1 Optional
6 BTY 706 Trends in Vaccinology 2+1 Optional
Total 1+2=3
SEMESTER II
7 RPE 700 Research and Publication Ethics* 1+1 Core
8 BTY 707 Advances in Bioinformatics 1+1 Optional
9 BTY 708 Advances in Reproductive Biotechnology 2+1 Optional
10 BTY 709 Advances in Animal Cell Culture* 2+1 Core
11 BTY 710 Industrial Biotechnology 2+1 Optional
Total 2+3=5
SEMESTER III
12 BTY 705 Gene Manipulation and Genome Editing 2+0 Optional
13 BTY 712 Doctorate Seminar-I* 1+0 Core
14 BTY 713 Doctorate Seminar-II 1+0 Core
15 BTY 714 Doctorate Research 0+15 Core
Total 4+15=19
SEMESTER IV
16 BTY 714 Doctorate Research 0+20 Core
Total 20
SEMESTER V
17 BTY 714 Doctorate Research 0+20 Core
Total 20
SEMESTER VI
18 BTY 714 Doctorate Research 0+20 Core
Total 20

5. Lecture Schedule – UG, PG, PhD – Theory / Practical Schedule – Approved by BoS –
Subject-wise

M.V.Sc. (Veterinary Biotechnology)

1. Course No.: BTY-601 Title: Basic & Applied Biotechnology (2+0)

Lecture No. Topic
UNIT I
1 History and scope of Biotechnology
2 – 3 Application of Biotechnology in agriculture and Veterinary Sciences, diagnostics and therapeutics, pharmaceutical industry, Food Industry and Chemical Industry, environmental sciences
4 Plant tissue culture and its applications
5 Biofermentation
UNIT II
6 - 7 Fermentation technology, aerobic and anaerobic fermentation
8 Different types of fermentation
9 Basic design and construction of fermenter
10 Upstream processing- Media sterilization, inoculum preparation
11 Downstream processing
12 – 13 Microbes and enzymes of industrial importance, Microbial growth kinetics and products.
UNIT III
14 Immobilized enzymes and cells
15 Immobilization process
16 - 17 Vaccines and their immune response
18 - 19 Types of vaccines - Conventional and new-generation vaccines
20 - 22 Subunit vaccine, recombinant vaccines
23 - 24 Vectored vaccines and DNA vaccine and their immune response
25 – 28 Edible vaccine, DIVA strategy and reverse vaccinology
UNIT IV
29 – 30 Biodiversity, genetic diversity, molecular taxonomy, species and population biodiversity
31 – 33 Quantifying biodiversity, maintenance of ecological diversity
34 - 36 Conservation of biodiversity and conservation of animal genetic resources

2. Course No.: BTY-602 Title: Fundamentals of Cell Biology (2+0)

Lecture No. Topic
UNIT I
1 - 2 Origin and evolution of cells from molecules to first cell from prokaryotes to eukaryotes from single to multicellular organisms
3 Chemical components of a cell Catalysis and use of energy by cells
4 - 7 Techniques used to study cells Principles and applications of microscopy, light microscopy, fluorescent microscopy, electron microscopy and confocal microscopy, Cell and cell parts separation techniques, Principles and applications of ultracentrifugation and flow cytometry, Detection of cell parts Primary and secondary antibodies used to detect cell parts
8 Structure of cell: Plasma membrane, Cytoskeleton
9 - 10 Structure of cell: Nucleus, Chromosome Chromosomal DNA packaging and its implications
11 Structure of cell: Endoplasmic reticulum, Ribosome Mitochondria
12 Structure of cell: Mitochondrial organization, Golgi complex Peroxisome, Lysosome
UNIT II
13 Cell Membrane transport: Transport of small molecules, Macromolecules and particles
14 Cell Membrane transport: Exocytosis and endocytosis Nuclear transport
15 Cell Membrane transport: Protein synthesis and sorting into Endoplasmic reticulum Golgi complex
16 - 17 Cell Membrane transport, Protein synthesis and sorting into Peroxisomes, Lysosomes, Lipid synthesis and sorting
18 - 19 Cell Membrane transport, Electron transport chain Chemiosmotic coupling
20 Transport of metabolites across the inner mitochondrial membrane
UNIT III
21 Mechanism of muscle contraction, cell crawling functions of keratin and neurofilaments
22 Cell Membrane transport: organelle transport, separation of mitotic chromosome
23 - 24 Cell signaling: Modes of cell-cell signaling, Steroid hormones and the steroid receptor super family
25 Cell signaling: Neurotransmitters, Peptide Hormones and Growth Factors
26 Cell signaling: G Protein-Coupled Receptors, Receptor Protein-Tyrosine Kinases
27 Cell signaling: Cytokine Receptors Non-Receptor Protein-Tyrosine Kinases
28 Cell signaling: The cAMP Pathway Second Messengers and Protein Phosphorylation
29 Cell signaling: Cyclic GMP Phospholipids and Ca2+
UNIT IV
30 - 31 Cell signaling: Ras, Raf, and the MAP Kinase Pathway The JAK/STAT Pathway, Integrins and Signal Transduction
32 – 33 Cell signaling: Regulation of the Actin Cytoskeleton Hedgehog and Wingless Notch Signaling
34 - 35 Cell signaling: Cell signal network, Feedback and crosstalk Networks of cellular signal transduction
36 Cell cycle, Regulators of cell cycle Events of M phase

3. Course No.: BTY 603 Title: Molecular Biology and Genetic Engineering (2+0)

Lecture No. Topic
UNIT I
1 History and scope of molecular biology – Discovery of DNA and evidence for DNA as the genetic material
2 - 3 Structure of DNA, RNA and proteins – Organization of prokaryotic and eukaryotic genome
4 Gene transfer in microorganisms like conjugation, transformation, transduction and protoplastic fusion
5 - 7 DNA replication - genetic code - transcription, RNA processing and alternative splicing
8 - 9 Translation in prokaryotes and eukaryotes - Regulation of gene expression
UNIT II
10 - 11 Enzymes used in molecular biology and recombinant DNA research
12 - 13 Cloning vectors – plasmids, phages, phagemids, cosmids, BAC, YAC
14 - 15 Expression vector – bacterial, viral, baculo and yeast vectors, shuttle vectors
16 - 17 Polymerase chain reaction and different types of PCR
18 - 19 Probes – Synthesis and types, Nucleic acid hybridization and blotting
20 Construction of gene libraries and cDNA library - Gene mapping and DNA structure analysis
UNIT III
21 - 24 Cloning in bacteria, yeast, plant and animal cells – identification of gene of interest and synthesis of double stranded DNA and complementary DNA
25 - 27 Restriction enzyme digestion – ligation - methods for transfer of cloned DNA - identification and enrichment of recombinant clones
28 - 29 Expression of recombinant DNA in prokaryotic and eukaryotic vectors - strategies for purification of expressed protein
UNIT IV
30 - 31 Molecular mechanism of mutation – DNA repair - site directed DNA alterations and gene manipulations
32 - 33 Gene editing techniques and Methods of DNA sequencing
34 Genetics of tumorigenic region of agrobacteria
35 Applications of genetic engineering in veterinary sciences
36 Ethics, legal issues and safety aspects of genetic manipulation

4. Course No.: BTY 604 Title: Animal Cell Culture – Principles and Application (2+1)
Theory

Lecture No. Topic
UNIT I
1 - 2 Introduction, History of cell culture development
3 - 4 Methods of sterilization
5 - 7 Tissue culture techniques- primary culture using various methods
8 - 10 Continuous cell lines- anchorage-dependent and independent cell lines
11 - 12 Organ culture
13 Cell bank and role of cell bank
UNIT II
14 - 17 Different types of cell culture media, Serum, growth supplements, balanced salt solution, Serum free media
18 - 19 Enzymes used in cell culture, Factors that affecting the growth of cells
UNIT III
20 Cell culture contaminants
21 Cryopreservation of primary culture and cell line
22 - 23 Cell cloning
24 - 26 Types of cell culture bioreactor
27 - 29 Cell counting and cytotoxic assays
UNIT IV
30 - 31 Applications of animal cell culture
32 - 33 Hybridoma technology and monoclonal antibody production
34 Applications of monoclonal antibodies in diagnostic and cancer research
35 - 36 Isolation and culturing of adult and embryonic stem cells and therapeutic applications of adult stem cells

Practical

Practical No. Topics
1 Packaging and sterilization of glass and plastic ware for cell culture
2 - 3 Preparation of reagents and media for cell culture
4 Primary chicken embryo fibroblast
5 Primary sheep/ goat kidney culture
6 - 8 Cultivation of continuous cell lines
9 Quantification of cells by trypan blue exclusion dye
10 - 11 Isolation of lymphocytes and cultivation
12 Study of effect of toxic chemicals on cultured mammalian cells
13 - 14 Study of cytopathic effect of virus on mammalian cells
15 - 16 Cryopreservation of primary cultures and cell lines
17 - 18 Isolation and culture of stem cells from bone marrow

5. Course No.: BTY 605 Title: Molecular Diagnostics (2+1)
Theory

Lecture No. Topics
UNIT I
1 - 2 Introduction, importance and historical perspective of development of molecular diagnostic technology
3 - 4 Development and optimization of nucleic acid detection assays: OIE guidelines
5 - 7 Concept of development of group specific and strain specific nucleic acid-based diagnostics, basis for selection of gene/ nucleotide sequence of pathogenic organism to target for detection
UNIT II
8 - 9 Types and application of different molecular diagnostic assays
10 - 11 Restriction endonuclease analysis for identification of pathogens
12 - 13 Principle of development of pathogen specific DNA probes Blotting techniques e.g. Southern and Northern hybridization
UNIT III
14 - 15 Nucleic Acid Sequence Based Amplification (NASBA)-Prospects and Applications
16 - 20 Historical background of development of PCR and other diagnostic assays, Signal, target and probe-based amplification techniques, Transcription based amplification (TBA)/ Nucleic Acid Sequence Based amplification (NASBA)/ Self-Sustaining Sequence Replication (SSSR/ 3SR), Strand Displacement Amplification (SDA), LAMP, Ligase chain reaction (LCR) - Prospects And Applications PCR principle, cyclic and thermal parameters in PCR, Real time PCR, Variations in PCR, application of PCR for diagnosis of infectious diseases of animals and poultry
21 - 22 Real-time PCR and its application in diagnosis
UNIT IV
23 Advancements in diagnostic technology platforms
24 DNA array technology
25 - 26 Nano - diagnostics
27 - 28 Biosensors
29 Mass spectrometry in disease diagnosis
30 - 31 Molecular cloning
32 - 33 Bead based assays
34 - 35 DNA sequencing including Next generation sequencing
36 Lateral-flow devices and its applications in diagnosis

Practical

Practical No. Topics
1 Preparation of buffers and reagents
2 - 3 Extraction of nucleic acids and qualitative and quantitative analysis of Nucleic acid
4 Agarose gel electrophoresis of Nucleic acids
5 - 6 Amplification of pathogen-specific gene using PCR
7 - 10 Different types of PCR including RT-PCR, nested PCR, etc
11 - 12 Real-time PCR
13 - 14 PCR - RFLP
15 - 16 DNA fingerprinting for identification of genetic diseases
17 - 18 Sanger sequencing using capillary electrophoresis

6. Course No.: BTY 606 Title: Immunology Applied to Biotechnology (2+1)
Theory

Lecture No. Topics
UNIT I
1 Introduction to principles of immunology, immune system and immune response
2 - 4 Major histocompatibility complex: its structure, functions and gene organization
5 - 6 MHC and its association with disease and resistance
7 Immunity against infectious agents of animals
8 - 9 Immunological tolerance
10 Autoimmunity: mechanism and control
11 - 12 Techniques used in biotechnology
UNIT II
13 - 14 Immunoglobulins and its type: Isotype, Allotype and Idiotype
15 - 16 Antibody production and purification
17 Application of antibodies in purification
18 - 19 Immunoblotting: principle and applications
20 - 21 Expression of immunoglobulin genes in plants and production of Antibodies
22 - 23 Cytokines: classification, structure, functions
24 Industrial production of cytokines and interferon
UNIT III
25 Application of antibodies in chemiluminescence and fluorescence assay for identification of recombinant genes
26 Antibody based nucleic acid probes and their applications
27 - 28 Immunoinformatics: concept and application
29 Transgenic animals and cellular chimeras
30 Immunodiagnostic tests: agar gel precipitation, agglutination reaction-based assays
31 Various types of Immunoassays, immunofiltration tests, flow cytometry in disease diagnosis
UNIT IV
32 Chimeric and humanized monoclonal antibodies
33 Recombinant antibodies: production and application
34 - 35 Modern uses of antibody: biosensors, catalysis, in vivo imaging, microarrays, proteomics
36 Cancer immunity and its immunotherapy

Practical

Practica l No. Topics
1 - 2 Agar gel immunodiffusion test; latex agglutination
3 Immunofiltration assay
4 Flow cytometry
5 Immunoelectrophoresis
6 - 7 Fluorescent antibody test
8 - 9 Enzyme immunoassays including various types of ELISA
10 Immunoblotting
11 Affinity chromatography
12 Lymphocyte proliferation assay
13 - 14 Cultivation of normal lymphocytes and myeloma cell line
15 - 16 Somatic cell hybridization and production of Hybridoma
17 Screening of hybrids for production of monoclonal antibodies
18 Bioinformatics tools for immunological research

7. Course No.: BTY 607 Title: Introduction to Bioinformatics (2+1)
Theory

Lecture No. Topics
UNIT I
1 - 4 Introduction to bioinformatics, concept and history of databases, various primary databases resources
5 Nucleic acid databases and their variants
6 Protein databases and its variants
7 Specialized genomic resources
UNIT II
8 - 9 DNA sequence analysis, introduction to concept indel, identity, mutations, gaps and penalties
10 - 12 cDNA library, its applications, EST, gene contigs, EST databases, EST analysis tools, sequence assembly tools and clustering EST libraries
13 Gene cloning vectors, their databases, tools and resources
14 Similarity vs homology, local and global alignments
15 - 18 Introduction to the concept of pair wise sequence alignment and multiple sequence alignment, difference between pair wise sequence alignment and multiple sequence alignment, introduction to various algorithms used in pair wise sequence alignment and multiple sequence alignments
19 - 20 Applications of phylogenetic analysis, type of phylogenetic trees
21 Introduction to methods/ matrixes used for construction of phylogenetic trees use of concept bootstrap value
UNIT III
22 Introduction to concept secondary database, their applications
23 Genome databases, animal genome databases
24 RNA database and their variants with applications
25 Building search protocols, use of search tools for homology/ similarity identification
26 - 27 Secondary protein databases, their applications, protein sequence structure relationship and patterns protein folding
28 - 29 Introduction to chemo-informatics and its applications, Applications of computer aided drug designing
30 Basic concept of computer aided drug designing
31 - 32 Structure based computer aided drug designing, ligand-based computer aided drug designing, databases searching, de novo drug designing
UNIT IV
33 Commercial databases and packages
34 GPL software for Bioinformatics
35 Web based analysis tools
36 Applications of bioinformatics in veterinary clinical research

Practical

Practica l No. Topics
1 - 2 Usage of NCBI resources, its variants and specialized databases
3 - 5 Retrieval of sequence/ structure from databases, retrieval of nucleic acid sequences and retrieval of protein sequence and structure studies
6 - 7 Protein's structure visualization, prediction using software and tools
8 - 9 Protein modelling
10 Protein antigenicity prediction tools
11 - 12 Using of ligand database tools and ligand docking
13 RNA database searching
14 - 15 BLAST searching tools generalized and specialized searches
16 - 17 Pair wise sequence alignment, multiple sequence alignment
18 Phylogenetic analysis

8. Course No.: BTY 608 Title: Animal Genomics (2+1)
Theory

Lecture No. Topics
UNIT I
1 - 3 Historical perspective, Genome organization in eukaryotes - Chromosome numbers in farm animals – Physical and molecular structure of chromosomes - Chromosome abnormalities in farm animals
4 - 5 High order structures, Role of cohesions and condensins in chromosome structure - SMC proteins
6 - 7 Importance of repetitive DNA – Classical satellites, microsatellites and mini satellites - SINES and LINES - Minisatellite and microsatellite-based fingerprinting techniques
UNIT II
8 - 9 Importance of gene mapping in livestock, methods and techniques used for gene mapping
10 - 11 Physical mapping - cytogenetic techniques, FISH technique in gene mapping
12 - 13 Gene mapping by somatic cell hybridization
14 - 15 Radiation hybrid maps for gene mapping
16 Linkage analysis - comparative gene mapping
UNIT III
17 - 18 DNA markers – Properties of DNA markers - RFLPs – Minisatellite and Microsatellite markers – PCR-based markers - RAPD, PCR-RFLPs, Allele specific – PCR, SSCP, STMS markers, DAMD-PCR, ARMS PCR, AP-PCR, RAMPO, AFLP, SNP, EST, etc.
19 Genetic characterization based on DNA markers, genetic distance analysis
20 Quantitative Trait Loci (QTL) - Candidate gene approach - QTL mapping Approach
21 Applications of DNA markers in livestock improvement - Marker Assisted Selection (MAS)
22 Marker Assisted Introgression – Parentage determination – SNP chips
23 Genomic selection based on SNP typing
24 - 25 Methods of genome editing – ZFN, TALENS, Meganucleases and CRISPR – Cas. Role of genome editing in livestock improvement
UNIT IV
26 Genome sequencing - Sanger Sequencing - Hierarchical shotgun approach
27 - 28 Next Generation Sequencing - Pyrosequencing - Semiconductor sequencing - Illumina sequencing - Helicos and SMRT sequencing platforms
29 - 30 Metagenomics – RNA Seq analysis
31 Exome sequencing and ddRAD sequencing for genome-wide SNP Detection
32 Current status of whole genome sequencing and gene maps of livestock
33 Role of MHC in disease resistance
34 Genes influencing production traits
35 Mitochondrial DNA of farm animals, evolutionary significance
36 Applications of genome analysis in animal breeding

Practical

Practica l No. Topics
1 - 2 Chromosome preparation (normal karyotyping, different types of banding) in farm animals
3 Isolation and purification of animal genomic DNA from blood lymphocytes
4 Analysis of DNA by agarose gel electrophoresis
5 Analysis of DNA by polyacrylamide gel electrophoresis
6 Checking the quality and quantity of genomic DNA by Spectrophotometer
7 - 8 Restriction digestion and analysis
9 Southern hybridization
10 DNA testing by microsatellite markers
11 - 12 Techniques for revealing polymorphism - PCR-RFLP
13 Single Strand Conformational Polymorphism (SSCP) analysis
14 AFLP, SNP chips
15 Genomic DNA cloning or cDNA cloning
16 Differentiation of tissues of different species by mitochondrial genome analysis
17 - 18 NGS data analysis - metagenome, RNASeq, exome and ddRAD sequence data by bioinformatics software

9. Course No.: BTY 609 Title: Techniques in Molecular Biology and Genetic Engineering
Practical (0+2)

Practica l No. Topics
1 - 2 Isolation of DNA from blood and mammalian cells
3 - 4 Isolation of bacterial plasmids
5 - 6 Restriction endonuclease digestion of plasmid and chromosomal DNA
7 - 8 Agarose gel electrophoresis of RE digested DNA
9 - 11 Polymerase Chain Reaction using random primers as well as specific primers
12 - 14 Different types of PCR
15 - 16 Isolation of mRNA/ RNA, Quantization of nucleic acids
17 - 19 cDNA synthesis
20 - 21 Real time polymerase chain reaction
22 - 23 Synthesis of nucleic acid probes and hybridization
24 - 26 Cloning of bacterial and viral genes into plasmid vectors
27 - 29 DNA ligation and transformation and confirmation of recombinants
30 - 31 Purification of recombinant proteins
32 - 34 Polyacrylamide gel electrophoresis (PAGE)
35 - 36 Western blot analysis

10. Course No.: BTY 610 Title: Reproductive Biotechnology (2+1)
Theory

Lectur e No. Topics
UNIT I
1 - 3 History, role of biotechnology in Assisted reproductive technology (ART)
4 - 5 Application of ART in human and animals
6 - 8 Multiple ovulation embryo transfer (MOET) - donor and recipient selection - synchronization - super ovulation - artificial insemination - embryo flushing - embryo evaluation - recipient management
UNIT II
9 - 11 Oocyte recovery from slaughterhouse ovaries and live animals, oocytes evaluation, and in-vitro maturation
12 - 14 In-vitro fertilization of oocytes, In-vitro culture and assessment of embryonic developmental stages
15 - 16 Micro assisted fertilization
17 - 18 Micromanipulation of gametes and embryos
19 Preservation of embryos and oocytes
UNIT III
20 - 22 Semen sexing technology and semen analysis
23 - 24 Embryo splitting
25 - 27 Different methods of embryo sexing
28 - 30 Transgenic animal production, application, limitation, and regulatory issues
UNIT IV
31 - 32 Somatic cell nuclear transfer of domestic animals and application
33 - 35 Isolation and characterization of embryonic stem cells
36 Different applications of embryonic stem cells

Practical

Practica l No. Topics
1 - 3 MOET protocols for domestic animals
4 - 5 Oocyte and embryo freezing protocol
6 - 9 Oocyte collection and evaluation from live and slaughterhouse animals
10 - 12 In-vitro embryo production
13 - 15 Embryo quality analysis
16 - 18 Embryo biopsy and embryo sexing

Ph.D. (Veterinary Biotechnology)

1. Course No.: BTY-701 Title: Genetic Engineering (1+2)
Theory

Lecture Nos. Topics
UNIT I
1 - 2 Cloning vectors- plasmids, phages, cosmids, BAC, YAC
3 - 4 Expression vectors- viral, baculo and yeast vectors, shuttle vectors
UNIT II
5 - 6 Restriction, ligation, transformation
7 Recombinant selection methods
8 - 9 Construction of genomic and cDNA library
10 - 11 Construction of full-length cDNA
12 Preparation of probe
13 Nick translation random hexamer and nick translation
UNIT III
14 Linkers, adapters, Cassettes
15 Screening the library
UNIT IV
16 - 17 Expressions of genes, prokaryotic and eukaryotic expression
18 Identification of protein, Purification of expressed protein

Practical

Practical Nos. Topics
1 - 2 Preparation of vector
3 - 4 Restriction enzyme digestion of vector
5 - 7 Preparation of target DNA and Purification of DNA
8 - 9 DNA ligation
10 - 12 Preparation of electro-competent cells
13 - 15 Transformation
16 - 18 Calculation of transformation efficiency
19 - 20 Screening by colony PCR
21 - 23 Selection of recombinant by insert release
24 - 26 Induction of expressed protein
27 - 32 Purification of expressed protein
33 - 34 SDS-PAGE
35 - 36 Western blotting

2. Course No.: BTY 702 Title: Functional Genomics and Proteomics (3+0)
Theory

Lectur e Nos. Topics
UNIT I
1 Overview of Mammalian Genome: Mitochondrial genome
2 Protein coding genes
3 - 4 RNA genes and repeat sequences
5 - 6 Variations in the mammalian genome
7 - 8 Expression of mammalian genome
UNIT II
9 - 10 Overview of Mammalian Transcriptome
11 - 13 Different methods to study gene expression
14 - 15 Single gene analysis, Northern blots, Quantitative PCR
16 - 18 SAGE, MPSS and SSH
19 - 20 Introduction to basic microarray technology, Design of experiments
21 - 22 Types of microarrays
UNIT III
23 - 24 Mammalian Genome - Chromosome number, evolution in mammalian species
25 Chromosome territory
26 - 27 Karyotyping, FISH and Spectral karyotyping
28 - 29 Next Generation sequencing platforms, chemistries and their applications
30 Mutation detection methods for single gene and genome-wide scale
UNIT IV
31 Databases such as NCBI, EBI
32 - 33 Nucleotide, Genome, SNP, Gene, Unigene, Homologene, Protein, etc. under NCBI. Service databases under EBI
34 - 35 Genome browsers, The concept of Comparative genomics, Genome BLAST and BLAT
36 - 38 Proteomics technology, identification and analysis of proteins by 2D analysis
39 - 40 Mass spectrophotometry
41 - 42 Circular Dichroism
43 - 44 Fluorescence Spectroscopy
45 - 46 NMR and X-ray crystallography
47 - 48 MALDI-TOF
49 - 50 Differential display proteomics
51 - 52 Protein-protein interaction, yeast two-hybrid system
53 - 54 Phage display

3. Course No.: BTY 703 Title: Advances in Cell and Molecular Biology (2+0)
Theory

Lecture Nos. Topics
UNIT I
1 Cell chemistry and biosynthesis pathway
2 Molecular motors of cell biology
3 Cell signaling
4 Signal transduction
5 Chemotropic energy metabolism
6 - 7 Apoptosis pathways
UNIT II
8 - 9 Structure and functions of prokaryotic and eukaryotic operons
10 - 11 Recombination and genetic variability
12 - 14 Regulation of gene expression
15 Strategies of nuclear transport
16 - 17 Carrier proteins and active membrane transport methodologies
UNIT III
18 Protein biosynthesis
19 - 20 Protein transportation
21 - 22 Protein sorting
23 Enzymes in molecular biology
24 - 25 Post-transcriptional control strategies
26 - 27 Plasmids in recombinant DNA technology
UNIT IV
28 - 30 RNA interference technology
31 - 32 Insights into nanobiology - Biosensors
33 DNA Microarray
34 - 35 Peptide Synthesis
36 Reverse Genetics

4. Course No.: BTY 704 Title: Diagnostic Platform (1+1)
Theory

Lectur e Nos. Topics
UNIT I
1 History and evolution of diagnostic platforms
2 Methods for identifying agents for infection or disease, point-of-care assays
3 Point-of-care assays
4 Principles for specific identification of the analytes or clinical parameters, various assays for different platforms
UNIT II
5 Cartridges, polymer cartridges - cartridge-based in-vitro diagnostics
6 - 7 Microfluidics/ nanotechnology sensors, complexity, and diversity of samples
8 - 10 Sample preparation, extraction of DNA/ RNA, PCR for marker DNA sequence
11 POC based on microfluidic chips
UNIT III
12 Detection principles - colorimetric, optical, electrochemical, magnetic, mechanical protein detection methods
13 Sensitive sensing principles - NASBA, RPA, LAMP with QUASR
14 Integrated microfluidic system
UNIT IV
15 Instrumentation for point-of-care diagnostic platform
16 Blood protein analyses, the Afinion platform from Axis-Shield
17 The Verigene® System by Nanosphere - Cepheid’s GeneXpert cassette
18 NorChip, use of smartphone apps for real-time monitoring and analysis

Practical

Practical Nos. Topics
1 - 3 DNA/ protein extraction
4 - 5 RNA extraction
6 - 7 Polymerase chain reaction
8 - 10 NASBA
11 - 13 RPA
14 - 15 LAMP
16 - 18 Microfluidic assay

5. Course No.: BTY 705 Title: Gene Manipulation and Genome Editing (2+0)
Theory

Lectur e Nos. Topics
UNIT I
1 Genetic architectures of model organisms: yeast, C. elegans, Drosophila, Mouse, human
2 Chromosomal and Genomic overviews of cattle, buffalo, yak, Mithun, sheep and goat
3 Chromosomal and Genomic overviews of pigs and poultry genome
UNIT II
4 - 5 Tools to characterize transgene: Identification and characterization of suitable transgene
6 - 7 Vectors used to clone and expression of foreign gene in prokaryotic systems
8 - 9 Vectors used to clone and expression of foreign gene in eukaryotic systems
10 - 11 Different types of promoters in prokaryotes and eukaryotes for tissue-specific expression of transgene
12 - 14 Detection of transgene in the new-born
UNIT III
15 - 16 Methods of Gene transfer: Microinjection of recombinant DNA into fertilized eggs/ stem cells
17 - 19 Transfection of DNA to totipotent/ ES cells and kerato-carcinoma cells
20 - 21 Electroporation, gene transfer into cultured mammalian cells
UNIT IV
22 - 23 Zinc finger and TALEN types and their mechanism
24 - 26 CRISPR types and their mechanism
27 - 28 Applications of these tools for in vivo genome engineering
29 - 30 Mono allelic and biallelic gene editing
31 - 32 Screening for genome editing process in cells/ animals
33 - 34 Applications of these tools in animal science for genetic studies, therapeutic potential and transgenic animal as bioreactors
35 - 36 Recent examples of genome-edited animals and their applications in animal science

6. Course No.: BTY 706 Title: Trends In Vaccinology (2+1)
Theory

Lectur e Nos. Topics
UNIT I
1 - 3 Immunity against veterinary infectious agents: bacteria, virus, fungi, and parasites
4 Immunoinformatics and its application to epitope mapping of pathogens, etc.
5 - 6 Advancement in vaccinology: Vaccinomics and Adversomics
7 - 8 Systems Vaccinology and Reverse vaccinology
UNIT II
9 Structural Vaccinology, computational vaccinology and its applications
10 Current trends in vaccine development against animal pathogens
11 - 13 Molecular approaches for vaccine development including: recombinant peptide vaccines, vectored vaccines, Marker vaccines, DNA vaccines, genetically manipulated live vaccines, etc.
14 Plant expression system-based vaccines
15 - 16 Idiotype and synthetic peptide-based vaccines
UNIT III
17 - 19 Vaccines and Immunotherapeutic for treating non-infectious diseases: Cancer; obesity, neurodegenerative diseases, addictions, atherosclerosis, etc.
20 DIVA Vaccines for animal disease
21 Vaccines for emerging human and animal diseases
22 - 23 Novel immunomodulators: Immunomodulators including cytokines and new adjuvants
24 - 26 Novel vaccine delivery systems: delivery of immunogens through liposomes, microspheres, ISCOMS, nanotechnology-based vaccine delivery, etc.
UNIT IV
27 Vaccine formulation: pharmacopeia requirements
28 Vaccine qualities and its control
29 Large scale vaccine production technology: cost-effectiveness of preventive immunization programmes
30 Stages of development of vaccine, clinical trials of vaccine, and its regulation
31 - 32 Commercial vaccines available against animal pathogens, its characteristics, and immunization schedule
33 Vaccine stability, preservation, and vaccination failure
34 Environmental concerns with the use of recombinant vaccines

Practical

Practical Nos. Topics
1 - 3 Purification of immunoglobulins: gel filtration and ion exchange chromatography
4 - 6 Hybridoma technique for monoclonal antibody production
7 - 8 Preparation of gene construct for recombinant and nucleic acid vaccine
9 - 12 Expression of gene encoding immunogenic protein in prokaryotic/yeast/animal cell culture system
13 - 14 Study of immune response against recombinant vaccine
15 Use of modern adjuvants in vaccines
16 - 18 Isolation and characterization of antigens from viruses, bacteria; Immunoassays: ELISA, FAT, RIA

7. Course No.: BTY 707 Title: Advances in Bioinformatics (1+1)
Theory

Lecture Nos. Topics
UNIT I
1 Introduction to Computational Gene Prediction and Genome Annotation
2 Basic concepts in Computational Phylogenetic Analysis, phylogenetic dating genome annotation; Gene networks
3 - 4 Completed genomes and bioinformatics approaches to analyze the genomes of Viruses, Bacteria, and Animals
UNIT II
5 - 6 Understanding of microarray data and correlation of gene expression data to biological processes and computational analysis tools
7 - 8 Methods of Genome sequencing, EST, STS, GSS database Whole Genome comparison
9 RNA folding, RNA loops, conformational study and specialized RNA databases
10 Whole genome analysis, whole genome regression, and prediction methods
UNIT III
11 - 12 Transcriptome analysis and its applications, Animal QTL databases and SIGENAE analysis of breeding animals genome
13 Tools of proteome analysis, Motifs and Folds; Protein structure-related databases, Protein Data Bank format, Concepts of B-factor and R-factor
14 Protein Structural Alignment and Superposition, Structure visualization of proteins, Protein Fold Classification, Protein structure comparison, CATH and SCOP Databases, Protein structure prediction methods, Homology modeling
15 - 16 Molecular Docking and Drug design (Basic concepts), Molecular dynamics and simulation study of protein, Force field concepts
UNIT IV
17 Protein identification and characterization
18 Introduction to the concept of chemoinformatics, metabolomics, and immunoinformatics

8. Course No.: BTY 708 Title: Advances in Reproductive Biotechnology (2+1)
Theory

Lecture Nos. Topics
Unit I
1-3 Micromanipulation of embryos and gametes, Somatic Cell Nuclear Transfer (SCNT), nuclear reprogramming
4-5 Transgenic animal production, Combining Transgenic and SCNT
6-7 Gene targeting
8-10 Genome editing and disease modeling
Unit II
11-12 In vivo Vs in-vitro production of embryos
13-16 Embryos quality, Transcriptomics, Metabolomic approach
17-18 Sperm seXing technologies and their application
19-20 Preimplantation genetic diagnosis and screening
Unit III
21-23 Epigenetic reprogramming
24-25 Large offspring syndrome
26-28 Sources of stem cells, Embryonic stem cells, Spermatogonial stem cells
29-30 Induced pluripotent stem cells
Unit IV
31-32 Stem cells application in regenerative medicine and disease therapeutics
33-34 Social, Ethical, Religious and regulatory issues related to assisted reproductive technology
35-36 Transgenic and stem cells therapy

Practical

Practica l Nos. Topics
1-3 Micromanipulator, micro assisted fertilization, ICSI protocol
4-6 Embryo biopsy - sexing
7-9 Sperm preparation and sperm quality analysis by flow cytometry
10-12 Embryo quality analysis - Morphological assessment and Staining technique
13-14 SCNT protocol - enucleation, somatic cell injection, fusion activation, and embryo culture
15-16 Isolation of inner cell mass from blastocyst, culture, and characterization of embryonic stem cells
17-18 Gene expression in sperm and embryos

9. Course No.: BTY 709 Title: Advances In Animal Cell Culture (2+1)
Theory

Lectur e Nos. Topics
Unit I
1-2 Development of cell lines using various methods
3-4 Characterization of cell lines by morphology
5-6 Characterization of cell lines by Chromosome analysis
7-9 Characterization of cell lines by DNA content, isoenzyme analysis, and antigenic markers
10-11 Characterization of cell lines by DNA fingerprinting
Unit II
12-15 Setting up a new cell culture lab
16-17 Detection methods for cell culture contaminants
18-20 Three-dimensional culture - classification of 3D culture methods and microfluidics
21-22 Tissue engineering - types of cells, Scaffold materials, Bioprinting, Bioartificial organs
Unit III
23-25 DNA transfer by viral and non-viral methods
26-28 Expression of recombinant proteins in mammalian and avian cell lines
29-31 Expression of recombinant proteins in mammalian and avian cell lines
Unit IV
32-34 Up-stream and downstream processing of cell culture-based vaccines, Diagnostic antigens, and other pharmaceutical agents
35-36 Cell culture fermenters

Practical

Practica l Nos. Topics
1 Primary and secondary mammalian cell culture
2-3 Development of transformed cells
4-5 Characterization of cell lines by karyotyping
6-7 Transfection of cells with recombinant DNA
8-9 Expression of recombinant proteins
10-11 Scaling-up of cultures
12 Flow Cytometry
13-14 Immunization of mice
15 Maintenance of myeloma cell lines
16 Fusion
17-18 Characterization of Mabs

10. Course No.: BTY 710 Title: Industrial Biotechnology (2+1)
Theory

Lecture Nos. Topics
Unit I
1 Introduction to fermentation process, history of fermentation process
2 Enzymes of industrial importance, Microbes producing industrially important enzymes
3-4 Screening of microbes for enzyme production
5 Genetic improvement of microorganism for improved production
6-7 Microbial growth studies and their metabolites-primary and secondary
8 Product formation by substrate degradation
9 Production of recombinant products
Unit II
10-11 Batch, continuous and fed batch fermentation
12-13 Kinetics of growth and product formation
14 Design of a fermenter, basic functions, types of fermenters
15 Animal cell culture by using bioreactors, Fermenters for animal cell culture
16-17 Aseptic operation, Containment, Sterilization
Unit III
18-19 Medium formulation, precursors, metabolic regulators, antifoams
20-21 Upstream and down stream processing
22 Filteration, Centrifugation
23-24 Extraction, Chromatography, membrane process
25-26 Drying crystallization, whole broth processing
Unit IV
27 Bioenergy production
28-30 Biohydrogen, biomethane, biodiesel and biobutanol production
31 Microbial fuel cells
32 Aerobic treatment of waste water
33 Anaerobic waste water treatment
34 Single cell protein production, Metal leaching
35 Food additives
36 Food supplements and health care products

Practical

Practical Nos. Topics
1-2 Isolation of industrially important enzyme producing microorganisms
3 Screening for enzyme production
4 Extraction and characterization of enzymes
5 Enzyme kinetics
6-7 Strain improvement by different methods
8-9 Bioreactor operation
10-11 Optimization of enzyme and industrial compounds production using bioreactor
12 Filtration
13 Cell disruption
14 Chromatography
15 Microbial fuel cell design
16 Optimization of electrodes, catholyte
17-18 Waste water treatment

11. BTY 711 Title: Rumen and Feed Biotechnology (2+1)
Theory

Lecture Nos. Topics
Unit I
1-2 Rumen ecosystem – Classification of rumen microbes
3-4 Isolation – Cultural characters of rumen microbes
5-6 Rumen fermentation - Techniques to increase production of rumen microbes
7-8 Metabolic inter-relationship between rumen microbes
Unit II
9-11 Feed processing and preservation, Microbial bioconversion of lignin and cellulose-rich feeds. Factors affecting delignification, Large-scale bioconversion of substrates
12-13 Pretreatment of feeds, chemical vs microbial treatment of feeds, Anti-nutritional factors present in feeds
14-15 Microbial detoxification of aflatoxins, Mimosine and other anti-metabolites present
Unit III
16-17 Manipulation of rumen methane production – Addition of methane inhibitors
18-20 Non-genetic manipulation of rumen microbes. Addition of antibiotics, Selective defaunation, Addition of fats. Addition of protein degradation protectants, Addition of buffer substances – Rumen escape proteins
Unit IV
21-22 Genetic manipulation of rumen microflora to improve feed utilization. Manipulation of rumen microbes by recombinant DNA technology
23-24 Inter-species H2 transfer and its importance – Single cell protein (SCP) as animal feed
25-26 Rumen metagenomics - Methods of studying rumen metagenome - Conventional cloning and sequencing of metagenomic DNA
27-28 NGS-based shotgun sequencing. Amplicon sequencing of 16S/18S rRNA hypervariable regions
29-30 Bioinformatics analysis of metagenomic sequence data
31-32 Use of probiotics. Microorganisms and proteins used as probiotics
33-34 Mechanism of action of probiotics, Immune response to probiotics
35-36 Anti-mutagenic and anti-tumor activities of probiotics

Practical

Practical Nos. Topics
1 Introduction to feeds and fodders for ruminants
2 Estimation of proximate principles in concentrates and roughages
3 Estimation of fibre fractions in concentrates and roughages
4 Methods for evaluating rumen fermentation parameters
5 Sampling of rumen contents – Microbial and protozoal count
6 Fixing and staining of rumen protozoa and bacteria
7 Estimation of rumen fermentation parameters-pH, Rumen NH3-N, Lactic acid
8 In-vitro Gas Production Test (IVGPT)
9 Rumen liquor analysis – Total volatile fatty acids – Individual volatile fatty acids – Ammonia Nitrogen
10 TCA precipitable Nitrogen-Methane production
11 Rumen microbial enzyme assay
12 Collection, isolation and quality check of DNA from rumen samples
13 Rumen metagenome and Bioinformatics analysis of metagenomic sequence data

12. Teaching Schedule: UG, PG, PhD – Prepared by – Course Teacher – Year wise / Course
Wise: Prepared as per the lecture schedule and Academic Calendar

13. Academic Calendar – UG, PG, PhD -Year wise / Semester Wise

14. College Classes Time Table: UG, PG, PhD – Year wise / Semester Wise

15. Examination Time Table – UG, PG, PhD – Semester / Year wise – Theory and Practical

16. Result –UG, PG, PhD – Semester Wise / Year Wise

Research:

1. List of Research Projects: Completed & Ongoing

Completed

Sr. No. Title of the Project Name of PI/ Major Advisor/ Research Worker Funding Agency Total Cost of the Project (Rs in Lakh) Extra-mural (Funding Agency)/ Intra-mural Status (Completed/ Ongoing) Duration
1 Qualitative Evaluation of the Silage prepared using Lactobacillus harbinensis PI: Dr. S. A. Ingle MAFSU Research Grants 2.00 Intra-mural Ongoing 01 year
2 Development of a Species-Specific Assay for Identification of Endangered Wild Canids Researcher: Barbuddhe Shruti S.
Advisor: Dr. S. A. Ingle		 M.V.Sc. research - - Ongoing 01 year
3 Expression of VP2 Protein of Feline Panleukopenia Virus for Diagnostic Application in Wild Felids Researcher: Kolangath Sujit M.
Advisor:
Dr. S. A. Ingle		 Ph. D. research - - Ongoing 03 years

2. List of Private Agency Projects: NA

Sr. No. Title of the Project Name of PI/ Major Advisor/ Research Worker Funding Agency Total Cost of the Project (Rs in Lakh) Extra-mural (Funding Agency)/ Intra-mural Status (Completed/ Ongoing) Duration

3. Research Priorities:

Sr. No. Research Area Aim Objective Benefits for Farmers
1 Development of Diagnostic Tools for Identification/ Detection of Infectious and/ or Non-Infectious Diseases To develop diagnostic tools Rapid detection of diseases Early diagnosis and treatment to prevent economic losses
2 Development of Molecular Tools for Identification of Different Species of Animals To identify the species of animals/animal origin samples using molecular tools Accurate identification of animal species from unidentifiable samples Application in molecular forensics
3 Evaluation and Improvement of the Nutritive Quality of Fermented Fodder To identify novel inoculums and evaluate the nutritional value of fermented fodder Find ways to preserve unconventional fodder crops and provide nutritive fodder Preservation of fodder by fermentation. Improved quality of fermented fodder

4. Thesis:
Master:

Name of Department: Veterinary Biotechnology
Sr. No. Name of the Student Title of Thesis Year of Completion Name of the Advisor
1 Nagargoje Rekha G. Phylogenetic analysis of Pseudomonas aeruginosa based on toxA and oprL genes and prediction of their immunogenic epitopes 2024 Dr. Sonal A. Ingle

Doctoral:

Name of Department:
Sr. No. Name of the Student Title of Thesis Year of Completion Name of the Advisor

5. Research Papers Published:
List of Research Publications:

Sr. No. Authors Year Title of the Research Paper Name of the Journal Volume; Page Nos. NAAS Rating, if any

List of Marathi and Popular articles published:

Sr. No. Authors Year Title Name of the Journal/Magazine Volume: Page Nos.

6. Research Recommendations

Sr. No.
Year
Project Title
Investigators
Recommendations

7. MOU signed by the Colleges

Sr. No. MOUs Between Party – 1 & Party - II Date of Signing Place Duration

8. Research Priorities / Thematic areas: Department Wise: Veterinary Biotechnology
Teaching and Research Cell

Sr. No. Title / Theme
1 Development of Diagnostic Tools for Identification/Detection of Infectious and/or non-infectious diseases
2 Development of Molecular Tools for Identification of Different Species of Animals
3 Evaluation and Improvement of the Nutritive Quality of Fermented Fodder

9. Significant Research Achievement:

Sr. No. Achievements Description
1 Patents Awarded
2 Breed Registration
3 IPR
4 Copyright
5 Trademark

Extension:

Field and other activities undertaken by faculty:

1. Training Organized

Farmers

Sr. No. Name of Training / Title of Training Duration (From…to…) No of Participants Name of Faculty

Officers

Sr. No. Name of Training Duration (From…to…) No of Participants Name of Faculty

2. Conference s / Workshop / Seminars / Webinars / Farmers meet organized

Farmers

Sr. No. Title of Workshop, Seminar, Farmers Meet Place Date No of Participants

Officers

Sr. No. Title of Conference / Seminar / Workshop Place Date No of Participants

3. Adopted/ Operational Villages

Sr. No. Name of Adopted Village Type of Extension Activity Brief Description (Guests, Resource Persons, Faculty Involved, No. of Participants)

Student Corner:

1. Awards / Recognitions:

Sr. No. Name of Student Name of Award / Achievement Place Date Awarding Agency

2. NSS: Regular / Special Camp (Year Wise)

Sr. No. Regular / Special No of Student Registered No. of Student Present Place Name of Activity Name of Expert

3. Photo Gallery (NSS )

Department:

Achievements of the Department: [Chronology as per the MAFSU Statute]

(PDF format)
1. No. of PG students graduated: 01
2. Research Publications:
3. Books/ Chapters: 02 chapters
4. No. of research schemes and agency funded projects completed:
5. No. of awards
6. Radio talks: 03
7. TV Talks
8. Marathi and Popular publications: 02
9. Any other achievement by the faculty and student

Faculty:

  • Name of Department: Veterinary Biotechnology Teaching and Research Cell
  • Brief History of Department:

The Animal Biotechnology Teaching and Research Cell was established at
Nagpur Veterinary College in 2007 under the aegis of the Department of Veterinary
Microbiology. The consistent and sincere efforts of Dr. D. R. Kalorey, Head of
Department of Veterinary Microbiology led to the introduction of a Master’s degree
program in Animal Biotechnology with the inception of the Animal Biotechnology T &
R Cell. Since then, the cell has been actively involved in P.G. Teaching and Research
activities. To date, 20 students have completed their Master’s in Animal Biotechnology.
Dr. Kalorey was the founder head (2007-2014) of the ABT T & R Cell followed
by Dr. V. C. Ingle (2014-2020) and Dr. S. R. Warke (2020-2024).
In 2024, the independent working of the Veterinary Biotechnology Teaching and
Research Cell was initiated. The PG program has been renamed Veterinary
Biotechnology as per the guidelines of ICAR-2021, along with the commencement of a
Ph.D. program in Veterinary Biotechnology. Currently, the Department is headed by Dr.
Sonal A. Ingle, Assistant Professor of biotechnology.
The cell aims: to meet the goals for PG and Ph.D. teaching and research; research to meet
the requirements of the livestock farmers, field personnel, industries, etc.; to provide
diagnostic facilities to the animal owners.

  • Laboratory Facilities in the Department:
  • Diagnostic services offered by the department:
  • Faculty: (Department Wise) – [ Chronology as per the MAFSU Statute ]

Academic officer:

Sr No Name of Academic Officer Qualification Designation Email ID Contact No Link to Digital Profile
1 Dr. Sonal A. Ingle Ph. D. (Animal Biotechnology) Assistant Professor sonalingle@mafsu.ac.in 8427057454

Academic staff:

Sr No Name of Academic Staff Qualification Designation Email ID Contact No Link to Digital Profile

Ministerial staff:

Sr No Name of Ministerial Staff Department Email
  • List of retired faculty: (Department wise)
Sr. No. Name of the Staff Designation Duration Photo
  • Photo gallery (Department Wise)